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首页> 外文期刊>Infection and immunity >Identification of LpeA, a PsaA-Like Membrane Protein That Promotes Cell Entry by Listeria monocytogenes
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Identification of LpeA, a PsaA-Like Membrane Protein That Promotes Cell Entry by Listeria monocytogenes

机译：LpeA的鉴定，一种类似PsaA的膜蛋白，可促进单核细胞增多性李斯特菌进入细胞

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The intracellular life of Listeria monocytogenes starts by a complex process of entry involving several bacterial ligands and eukaryotic receptors. In this work, we identified in silico from the sequence of the genome of L. monocytogenes a previously unknown gene designated lpeA (for lipoprotein promoting entry) encoding a 35-kDa protein homologous to PsaA, a lipoprotein belonging to the LraI family and implicated in the cell adherence of Streptococcus pneumoniae and related species. By constructing a mutant of L. monocytogenes in which lpeA is deleted (lpeA mutant), we show that the PsaA-like protein LpeA is not involved in bacterial adherence but is required for entry of L. monocytogenes in eukaryotic cells. In contrast to wild-type bacteria, mutant bacteria failed to invade the epithelial Caco-2 and hepatocyte TIB73 cell lines, as confirmed by confocal microscopy. The mutant bacteria rapidly penetrated in mouse bone marrow-derived macrophages. Surprisingly, lpeA mutant bacteria survive better in macrophages than do wild-type bacteria. This was correlated with a weak exacerbation of virulence of the lpeA mutant in the mouse. LpeA is therefore a novel invasin favoring the entry of L. monocytogenes into nonprofessional phagocytes but not its invasion of macrophages. This is the first report of a lipoprotein promoting cell invasion of an intracellular pathogen.
机译：单核细胞增生李斯特菌的细胞内生活始于一个复杂的进入过程，涉及多个细菌配体和真核受体。在这项工作中，我们从 L的基因组序列中鉴定了真核生物。单核细胞增生病一个以前未知的基因，称为 lpeA （用于促进脂蛋白进入），编码与PsaA同源的35 kDa蛋白，PsaA是属于LraI家族的脂蛋白，与的细胞粘附有关>肺炎链球菌及相关种。通过构建 L的突变体。缺失 lpeA （ lpeA 突变体）的单核细胞增生，我们发现PsaA样蛋白LpeA不参与细菌粘附，但是进入L。真核细胞中的单核细胞增生通过共聚焦显微镜证实，与野生型细菌相反，突变细菌无法侵入上皮Caco-2和肝细胞TIB73细胞系。突变细菌迅速渗透到小鼠骨髓来源的巨噬细胞中。令人惊讶的是，与野生型细菌相比， lpeA 突变细菌在巨噬细胞中存活得更好。这与 lpeA 突变体在小鼠中的毒力弱加重有关。因此，LpeA是有利于 L进入的新的入侵蛋白。单核细胞增生进入非专业吞噬细胞，但不侵袭巨噬细胞。这是脂蛋白促进细胞侵袭细胞内病原体的首次报道。

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