...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Age-Related Resistance of C57BL/6 Mice to Cryptococcus neoformans Is Dependent on Maturation of NKT Cells
【24h】

Age-Related Resistance of C57BL/6 Mice to Cryptococcus neoformans Is Dependent on Maturation of NKT Cells

机译：C57BL / 6小鼠对新型隐球菌的年龄相关抗性取决于NKT细胞的成熟度。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Conflicting results have been reported regarding the ability of C57BL/6 mice to clear infections due to Cryptococcus neoformans. Examination of the various experimental protocols used suggested that C57BL/6 mice might develop the ability to resist infection as they mature. We analyzed the ability of C57BL/6 mice of different ages to respond to immunization with cryptococcal antigen or to clear a cryptococcal infection. Mice were immunized with a soluble cryptococcal culture filtrate antigen (CneF) emulsified in complete Freund's adjuvant (CneF-CFA). Delayed-type hypersensitivity (DTH) reactions elicited by the immunization were significantly stronger in 15-week-old C57BL/6 mice than in 7-week-old mice. Analysis of cryptococcal CFU 8 weeks following intratracheal infection of 7-week-old mice or 15-week-old mice revealed a relative inability of the younger animals to control the infection. Six-week-old immunized and infected mice cleared cryptococci from brain, spleen, and liver in a manner similar to that of immunized and infected 15-week-old mice. However, the older mice cleared cryptococci much more efficiently from the lungs. The possible role for NKT cells was determined by passive transfer of thymocytes from 10-week-old mice (containing mature NKT cells) or 2-week-old mice (containing immature NKT cells) to 6-week-old mice. The 10-week-old thymocytes significantly enhanced the ability of the mice to develop a DTH response after immunization with CneF-CFA, while animals treated with 2-week-old thymocytes did not improve their DTH response after immunization. The cells in the 10-week-old thymocyte population responsible for improvement of DTH responses were identified as being NK1.1 positive.
机译：据报道，关于C57BL / 6小鼠清除新隐球菌引起的感染的能力存在矛盾。对各种实验方案的检查表明，C57BL / 6小鼠在成熟时可能会发展出抵抗感染的能力。我们分析了不同年龄的C57BL / 6小鼠对隐球菌抗原免疫反应或清除隐球菌感染的能力。用在完全弗氏佐剂（CneF-CFA）中乳化的可溶性隐球菌培养物滤液抗原（CneF）免疫小鼠。免疫引起的迟发型超敏反应（DTH）在15周龄的C57BL / 6小鼠中明显强于7周龄的小鼠。气管内感染7周龄或15周龄小鼠后8周对隐球菌CFU的分析显示，较年轻的动物相对无法控制感染。六周龄的免疫和感染小鼠以与免疫和感染的15周龄小鼠类似的方式从大脑，脾脏和肝脏清除隐球菌。但是，年长的小鼠从肺部清除隐球菌的效率更高。通过将胸腺细胞从10周龄小鼠（包含成熟NKT细胞）或2周龄小鼠（包含未成熟NKT细胞）被动转移到6周龄小鼠，来确定NKT细胞的可能作用。用CneF-CFA免疫后，10周龄的胸腺细胞显着增强了小鼠产生DTH应答的能力，而用2周龄的胸腺细胞处理的动物在免疫后并未改善其DTH应答。 10周大的胸腺细胞群中负责DTH反应改善的细胞被鉴定为NK1.1阳性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号