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首页> 外文期刊>Infection and immunity >Transcriptomic and Innate Immune Responses to Yersinia pestis in the Lymph Node during Bubonic Plague
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Transcriptomic and Innate Immune Responses to Yersinia pestis in the Lymph Node during Bubonic Plague

机译：鼠疫鼠疫淋巴结中的鼠疫耶尔森菌的转录组和先天免疫应答。

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A delayed inflammatory response is a prominent feature of infection with Yersinia pestis, the agent of bubonic and pneumonic plague. Using a rat model of bubonic plague, we examined lymph node histopathology, transcriptome, and extracellular cytokine levels to broadly characterize the kinetics and extent of the host response to Y. pestis and how it is influenced by the Yersinia virulence plasmid (pYV). Remarkably, dissemination and multiplication of wild-type Y. pestis during the bubonic stage of disease did not induce any detectable gene expression or cytokine response by host lymph node cells in the developing bubo. Only after systemic spread had led to terminal septicemic plague was a transcriptomic response detected, which included upregulation of several cytokine, chemokine, and other immune response genes. Although an initial intracellular phase of Y. pestis infection has been postulated, a Th1-type cytokine response associated with classical activation of macrophages was not observed during the bubonic stage of disease. However, elevated levels of interleukin-17 (IL-17) were present in infected lymph nodes. In the absence of pYV, sustained recruitment to the lymph node of polymorphonuclear leukocytes (PMN, or neutrophils), the major IL-17 effector cells, correlated with clearance of infection. Thus, the ability to counteract a PMN response in the lymph node appears to be a major in vivo function of the Y. pestis virulence plasmid.
机译：延缓的炎症反应是感染鼠疫耶尔森菌的重要特征，鼠疫耶尔森氏菌是鼠疫和肺鼠疫的病原体。使用鼠疫鼠模型，我们检查了淋巴结的组织病理学，转录组和细胞外细胞因子水平，以广泛表征宿主对 Y的反应动力学和程度。鼠疫及其受耶尔森氏菌毒力质粒（pYV）的影响。值得注意的是，野生型 Y的传播和繁殖。在疾病的发病期，鼠疫没有诱导发育中的腹股沟淋巴瘤宿主淋巴结细胞检测到任何可检测的基因表达或细胞因子反应。只有在全身扩散导致最终的败血性瘟疫之后，才检测到转录组反应，其中包括几种细胞因子，趋化因子和其他免疫反应基因的上调。尽管初始细胞内阶段为Y。据推测，瘟疫感染是在疾病的发病期未观察到与巨噬细胞经典激活相关的Th1型细胞因子反应。但是，在感染的淋巴结中存在高水平的白介素-17（IL-17）。在没有pYV的情况下，持续的募集到主要的IL-17效应细胞多形核白细胞（PMN或嗜中性粒细胞）的淋巴结与感染清除率相关。因此，抵抗淋巴结PMN反应的能力似乎是 Y的主要体内功能。鼠疫毒力质粒。

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