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首页> 外文期刊>Infection and immunity >Cooperative Role for Tetraspanins in Adhesin-Mediated Attachment of Bacterial Species to Human Epithelial Cells
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Cooperative Role for Tetraspanins in Adhesin-Mediated Attachment of Bacterial Species to Human Epithelial Cells

机译：四跨膜蛋白在粘附素介导的细菌物种对人上皮细胞的附着中的合作作用。

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The tetraspanins are a superfamily of transmembrane proteins with diverse functions and can form extended microdomains within the plasma membrane in conjunction with partner proteins, which probably includes receptors for bacterial adhesins. Neisseria meningitidis, the causative agent of meningococcal disease, attaches to host nasopharyngeal epithelial cells via type IV pili and opacity (Opa) proteins. We examined the role of tetraspanin function in Neisseria meningitidis adherence to epithelial cells. Tetraspanins CD9, CD63, and CD151 were expressed by HEC-1-B and DETROIT 562 cells. Coincubation of cells with antibodies against all three tetraspanin molecules used individually or in combination, with recombinant tetraspanin extracellular domains (EC2), or with small interfering RNAs (siRNAs) significantly reduced adherence of Neisseria meningitidis. In contrast, recombinant CD81, a different tetraspanin, had no effect on meningococcal adherence. Antitetraspanin antibodies reduced the adherence to epithelial cells of Neisseria meningitidis strain derivatives expressing Opa and pili significantly more than isogenic strains lacking these determinants. Adherence to epithelial cells of strains of Staphylococcus aureus, Neisseria lactamica, Escherichia coli, and Streptococcus pneumoniae was also reduced by pretreatment of cells with tetraspanin antibodies and recombinant proteins. These data suggest that tetraspanins are required for optimal function of epithelial adhesion platforms containing specific receptors for Neisseria meningitidis and potentially for multiple species of bacteria.
机译：四跨膜蛋白是具有多种功能的跨膜蛋白超家族，可与伴侣蛋白（可能包括细菌粘附素的受体）一起在质膜内形成扩展的微区。脑膜炎奈瑟氏球菌是脑膜炎球菌病的病原体，通过IV型菌毛和不透明（Opa）蛋白附着在宿主鼻咽上皮细胞上。我们检查了四跨膜蛋白功能在脑膜炎奈瑟氏球菌对上皮细胞的粘附中的作用。四跨素CD9，CD63和CD151由HEC-1-B和DETROIT 562细胞表达。将细胞与针对所有三种单独或组合使用的四跨膜蛋白分子的抗体，重组四跨膜蛋白胞外域（EC2）或小干扰RNA（siRNA）共同孵育，可显着降低脑膜炎奈瑟氏菌的粘附。相反，重组CD81，一种不同的四跨膜蛋白，对脑膜炎球菌的粘附没有影响。与缺乏这些决定簇的同基因菌株相比，抗四跨膜蛋白抗体显着降低了表达Opa和菌毛的脑膜炎奈瑟氏菌菌株衍生物对上皮细胞的粘附。通过用四跨膜蛋白抗体和重组蛋白预处理细胞，还可以减少金黄色葡萄球菌，乳酸奈瑟菌，大肠杆菌和肺炎链球菌菌株对上皮细胞的粘附。这些数据表明，四跨膜蛋白是上皮粘附平台的最佳功能所必需的，该平台包含脑膜炎奈瑟氏球菌以及多种细菌的特异性受体。

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