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首页> 外文期刊>Infection and immunity >Characterization of a Staphylococcus aureus Surface Virulence Factor That Promotes Resistance to Oxidative Killing and Infectious Endocarditis
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Characterization of a Staphylococcus aureus Surface Virulence Factor That Promotes Resistance to Oxidative Killing and Infectious Endocarditis

机译：表征金黄色葡萄球菌表面毒力因子，促进对氧化性杀伤和传染性心内膜炎的抵抗力

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Staphylococcus aureus is a prominent human pathogen and a leading cause of community- and hospital-acquired bacterial infections worldwide. Herein, we describe the identification and characterization of the S. aureus 67.6-kDa hypothetical protein, named for the surface factor promoting resistance to oxidative killing (SOK) in this study. Sequence analysis showed that the SOK gene is conserved in all sequenced S. aureus strains and homologous to the myosin cross-reactive antigen of Streptococcus pyogenes. Immunoblotting and immunofluorescence analysis showed that SOK was copurified with membrane fractions and was exposed on the surface of S. aureus Newman and RN4220. Comparative analysis of wild-type S. aureus and an isogenic deletion strain indicated that SOK contributes to both resistance to killing by human neutrophils and to oxidative stress. In addition, the S. aureus sok deletion strain showed dramatically reduced aortic valve vegetation and bacterial cell number in a rabbit endocarditis model. These results, plus the suspected role of the streptococcal homologue in certain diseases such as acute rheumatic fever, suggest that SOK plays an important role in cardiovascular and other staphylococcal infections.
机译：金黄色葡萄球菌是一种重要的人类病原体，也是全球社区和医院获得性细菌感染的主要原因。在本文中，我们描述了金黄色葡萄球菌67.6-kDa假定蛋白的鉴定和表征，该蛋白在本研究中以促进抗氧化杀伤（SOK）的表面因子命名。序列分析表明，SOK基因在所有测序的金黄色葡萄球菌菌株中都是保守的，并且与化脓性链球菌的肌球蛋白交叉反应抗原同源。免疫印迹和免疫荧光分析表明SOK与膜级分共纯化，并暴露在金黄色葡萄球菌Newman和RN4220的表面上。对野生型金黄色葡萄球菌和一个等基因缺失菌株的比较分析表明，SOK既有助于抵抗人类嗜中性粒细胞的杀伤，又有助于抵抗氧化应激。另外，在兔心内膜炎模型中，金黄色葡萄球菌Sok缺失菌株显示出主动脉瓣植被和细菌细胞数量的显着减少。这些结果，加上链球菌同系物在某些疾病（如急性风湿热）中的怀疑作用，表明SOK在心血管和其他葡萄球菌感染中起重要作用。

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