...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Identification of Salmonella enterica Serovar Pullorum Antigenic Determinants Expressed In Vivo
【24h】

Identification of Salmonella enterica Serovar Pullorum Antigenic Determinants Expressed In Vivo

机译：体内表达的肠炎沙门氏菌血清白蛋白抗原决定簇的鉴定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Salmonella enterica serovar Pullorum affecting poultry causes pullorum disease and results in severe economic loss in the poultry industry. Currently, it remains a major threat in countries with poor poultry surveillance and no efficient control measures. As S. Pullorum could induce strong humoral immune responses, we applied an immunoscreening technique, the in vivo-induced antigen technology (IVIAT), to identify immunogenic bacterial proteins expressed or upregulated during S. Pullorum infection. Convalescent-phase sera from chickens infected with S. Pullorum were pooled, adsorbed against antigens expressed in vitro, and used to screen an S. Pullorum genomic expression library. Forty-five proteins were screened out, and their functions were implicated in molecular biosynthesis and degradation, transport, metabolism, regulation, cell wall synthesis and antibiotic resistance, environmental adaptation, or putative functions. In addition, 11 of these 45 genes were assessed for their differential expression by quantitative real-time reverse transcription-PCR (RT-PCR), revealing that 9 of 11 genes were upregulated to different degrees under in vivo conditions, especially the regulator of virulence determinants, phoQ. Then, four in vivo-induced proteins (ShdA, PhoQ, Cse3, and PbpC) were tested for their immunoreactivity in 28 clinical serum samples from chickens infected with S. Pullorum. The rate of detection of antibodies against ShdA reached 82% and was the highest among these proteins. ShdA is a host colonization factor known to be upregulated in vivo and related to the persistence of S. Typhimurium in the intestine. Furthermore, these antigens identified by IVIAT warrant further evaluation for their contributions to pathogenesis, and more potential roles, such as diagnostic, therapeutic, and preventive uses, need to be developed in future studies.
机译：感染家禽的肠炎沙门氏菌引起的白痢沙门氏菌会引起白痢病，并在家禽业造成严重的经济损失。当前，它仍然是家禽监测不力，没有有效控制措施的国家的主要威胁。由于S. Pullorum可以诱导强烈的体液免疫反应，因此我们应用了一种免疫筛选技术，即体内诱导抗原技术（IVIAT），以鉴定在S. Pullorum感染期间表达或上调的免疫原性细菌蛋白。合并感染白痢菌的鸡的恢复期血清，吸附体外表达的抗原，并用于筛选白痢菌基因组表达文库。筛选出45种蛋白质，其功能与分子生物合成和降解，转运，代谢，调节，细胞壁合成和抗生素抗性，环境适应性或推定功能有关。此外，通过定量实时逆转录PCR（RT-PCR）评估了这45个基因中的11个基因的差异表达，揭示了11个基因中的9个在体内条件下被上调到不同程度，尤其是毒力调节剂行列式，phoQ。然后，测试了四种体内诱导的蛋白（ShdA，PhoQ，Cse3和PbpC）在来自感染鸡白痢的鸡的28种临床血清样品中的免疫反应性。抗ShdA抗体的检测率达到82％，是这些蛋白中最高的。 ShdA是已知在体内上调的宿主定殖因子，与鼠伤寒沙门氏菌在肠道中的持久性有关。此外，通过IVIAT鉴定的这些抗原需要进一步评估其对发病的贡献，并且在未来的研究中还需要开发更多潜在的作用，例如诊断，治疗和预防用途。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号