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首页> 外文期刊>Infection and immunity >The Secreted Esterase of Group A Streptococcus Is Important for Invasive Skin Infection and Dissemination in Mice
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The Secreted Esterase of Group A Streptococcus Is Important for Invasive Skin Infection and Dissemination in Mice

机译：A组链球菌的分泌酯酶对小鼠的侵袭性皮肤感染和传播很重要

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Virulence factors regulated by the CovRS/CsrRS two-component gene regulatory system contribute to the invasive diseases caused by group A Streptococcus (GAS). To determine whether the streptococcal secreted esterase (Sse), an antigen that protects against subcutaneous GAS infection, is one of these virulence factors, we investigated the phenotype of a nonpolar sse deletion mutant strain (Δsse). In addition, we examined the effects of covS mutation on sse expression. As assessed using a mouse model of subcutaneous infection, the virulence of the Δsse strain is attenuated and the overall pathology is reduced. Furthermore, GAS was detected in the blood and spleens from mice subcutaneously infected with the parental strain, whereas mice subcutaneously infected with the Δsse strain had no GAS present in their blood and spleens. The ability of the mutant to survive in the subcutis of mice appeared to be compromised. The growth of the Δsse strain in rich and chemically defined media and nonimmune human blood and sera was slower than that of the wild-type strain. Complementation restored the phenotype of the Δsse strain to that of the wild-type strain. The wild-type, Δsse, and complement strains had no detectable SpeB activity. Expression of Sse is negatively controlled by CovRS. These findings suggest that Sse is a CovRS-regulated virulence factor that is important for the virulence of GAS in subcutaneous infection and plays an important role in severe soft tissue infections and systemic dissemination of GAS from the skin.
机译：CovRS / CsrRS两组分基因调控系统调控的毒力因子可导致由A组链球菌（GAS）引起的侵袭性疾病。为了确定分泌 s 链球菌 s 分泌的 e 酯酶（Sse）（一种防止皮下GAS感染的抗原）是否为这些毒力因子之一，我们研究了非极性 sse 缺失突变株（Δ sse ）的表型。此外，我们研究了 covS 突变对 sse 表达的影响。如使用皮下感染的小鼠模型评估的，Δ sse 菌株的毒力减弱，总体病理降低。此外，皮下感染了亲本菌株的小鼠的血液和脾脏中均检测到了GAS，而Δ sse 菌株皮下感染的小鼠的血液和脾脏中均未检测到GAS。突变体在小鼠皮下存活的能力似乎受到损害。 Δ sse 菌株在丰富和化学成分确定的培养基以及非免疫性人血和血清中的生长比野生型菌株慢。互补使Δ sse 菌株的表型恢复为野生型菌株的表型。野生型Δ sse 和补体菌株均未检测到SpeB活性。 Sse的表达受CovRS负控制。这些发现表明，Sse是CovRS调节的毒力因子，对于皮下感染中GAS的毒力很重要，并且在严重的软组织感染和GAS从皮肤的全身扩散中起重要作用。

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