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Comparative Analysis of the Locus of Enterocyte Effacement and Its Flanking Regions

机译：肠上皮细胞形成部位及其侧翼区域的比较分析

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The attaching-and-effacing (A/E) phenotype mediated by factors derived from the locus of enterocyte effacement (LEE) is a hallmark of clinically important intestinal pathotypes of Escherichia coli, including enteropathogenic (EPEC), atypical EPEC (ATEC), and enterohemorrhagic E. coli strains. Epidemiological studies indicate that the frequency of diarrhea outbreaks caused by ATEC is increasing. Hence, it is of major importance to further characterize putative factors contributing to the pathogenicity of these strains and to gain additional insight into the plasticity and evolutionary aspects of this emerging pathotype. Here, we analyzed the two clinical ATEC isolates B6 (O26:K60) and 9812 (O128:H2) and compared the genetic organizations, flanking regions, and chromosomal insertion loci of their LEE with those of the LEE of other A/E pathogens. Our analysis shows that the core LEE is largely conserved—particularly among genes coding for the type 3 secretion system—whereas genes encoding effector proteins display a higher variability. Chromosomal insertion loci appear to be restricted to selC, pheU, and pheV. In contrast, striking differences were found between the 5′- and 3′-associated flanking regions reflecting the different histories of the various strains and also possibly indicating different lines in evolution.
机译：由肠上皮细胞侵染（LEE）的部位所介导的因子介导的附着和表皮（A / E）表型是大肠杆菌在临床上重要的肠道病理类型的标志，包括肠道致病性（EPEC），非典型EPEC（ATEC）和肠出血性 E。大肠杆菌菌株。流行病学研究表明，由ATEC引起的腹泻暴发频率正在增加。因此，进一步表征促成这些菌株致病性的推定因素并获得对该新兴病态的可塑性和进化方面的进一步了解至关重要。在这里，我们分析了两个临床ATEC分离株B6（O26：K60）和9812（O128：H2），并比较了其LEE与其他A / E病原体的LEE的遗传组织，侧翼区域和染色体插入位点。我们的分析表明，核心LEE分子在很大程度上是保守的，特别是在编码3型分泌系统的基因中，而编码效应蛋白的基因则表现出更高的变异性。染色体插入基因座似乎仅限于 selC ， pheU 和 pheV 。相反，在5'和3'相关的侧翼区域之间发现了显着差异，这反映了各种菌株的不同历史，并且还可能指示进化过程中的不同系。

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