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首页> 外文期刊>Infection and immunity >The Acinetobacter baumannii 19606 OmpA Protein Plays a Role in Biofilm Formation on Abiotic Surfaces and in the Interaction of This Pathogen with Eukaryotic Cells
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The Acinetobacter baumannii 19606 OmpA Protein Plays a Role in Biofilm Formation on Abiotic Surfaces and in the Interaction of This Pathogen with Eukaryotic Cells

机译：鲍曼不动杆菌19606 OmpA蛋白在非生物表面生物膜形成以及这种病原体与真核细胞的相互作用中发挥作用

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The ability of Acinetobacter baumannii to adhere to and persist on surfaces as biofilms could be central to its pathogenicity. The production of pili and a biofilm-associated protein and the expression of antibiotic resistance are needed for robust biofilm formation on abiotic and biotic surfaces. This multistep process also depends on the expression of transcriptional regulatory functions, some of which could sense nutrients available to cells. This report extends previous observations by showing that although outer membrane protein A (OmpA) of A. baumannii 19606 plays a partial role in the development of robust biofilms on plastic, it is essential for bacterial attachment to Candida albicans filaments and A549 human alveolar epithelial cells. In contrast to abiotic surfaces, the interaction with biotic surfaces is independent of the CsuA/BABCDE-mediated pili. The interaction of A. baumannii 19606 with fungal and epithelial cells also results in their apoptotic death, a response that depends on the direct contact of bacteria with these two types of eukaryotic cells. Furthermore, the bacterial adhesion phenotype correlates with the ability of bacteria to invade A549 epithelial cells. Interestingly, the killing activity of cell-free culture supernatants proved to be protease and temperature sensitive, suggesting that its cytotoxic activity is due to secreted proteins, some of which are different from OmpA.
机译：鲍曼不动杆菌附着并持久存在于表面的能力，因为生物膜可能是其致病性的关键。菌毛和生物膜相关蛋白的产生以及抗生素抗性的表达是在非生物和生物表面牢固形成生物膜所必需的。这个多步骤过程还取决于转录调节功能的表达，其中一些可以感知细胞可利用的营养。该报告通过显示 A的外膜蛋白A（OmpA）扩展了先前的观察结果。 baumannii 19606在塑料上牢固的生物膜的形成中起部分作用，这对于细菌附着于白色念珠菌丝和A549人肺泡上皮细胞至关重要。与非生物表面相反，与生物表面的相互作用独立于CsuA / BABCDE介导的菌毛。 A的交互作用。鲍曼氏菌19606与真菌和上皮细胞也会导致其凋亡死亡，这种反应取决于细菌与这两种类型的真核细胞的直接接触。此外，细菌粘附表型与细菌入侵A549上皮细胞的能力有关。有趣的是，无细胞培养上清液的杀伤活性被证明对蛋白酶和温度敏感，表明其细胞毒性活性是由于分泌的蛋白质引起的，其中一些蛋白质不同于OmpA。

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