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首页> 外文期刊>Infection and immunity >Intrastrain Heterogeneity of the mgpB Gene in Mycoplasma genitalium Is Extensive In Vitro and In Vivo and Suggests that Variation Is Generated via Recombination with Repetitive Chromosomal Sequences
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Intrastrain Heterogeneity of the mgpB Gene in Mycoplasma genitalium Is Extensive In Vitro and In Vivo and Suggests that Variation Is Generated via Recombination with Repetitive Chromosomal Sequences

机译：生殖器支原体中mgpB基因的株内异质性在体内和体外广泛存在，并表明变异是通过与重复染色体序列的重组产生的。

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Mycoplasma genitalium is associated with reproductive tract disease in women and may persist in the lower genital tract for months, potentially increasing the risk of upper tract infection and transmission to uninfected partners. Despite its exceptionally small genome (580 kb), approximately 4% is composed of repeated elements known as MgPar sequences (MgPa repeats) based on their homology to the mgpB gene that encodes the immunodominant MgPa adhesin protein. The presence of these MgPar sequences, as well as mgpB variability between M. genitalium strains, suggests that mgpB and MgPar sequences recombine to produce variant MgPa proteins. To examine the extent and generation of diversity within single strains of the organism, we examined mgpB variation within M. genitalium strain G-37 and observed sequence heterogeneity that could be explained by recombination between the mgpB expression site and putative donor MgPar sequences. Similarly, we analyzed mgpB sequences from cervical specimens from a persistently infected woman (21 months) and identified 17 different mgpB variants within a single infecting M. genitalium strain, confirming that mgpB heterogeneity occurs over the course of a natural infection. These observations support the hypothesis that recombination occurs between the mgpB gene and MgPar sequences and that the resulting antigenically distinct MgPa variants may contribute to immune evasion and persistence of infection.
机译：生殖道支原体与女性生殖道疾病有关，可能在下生殖道中持续数月，可能增加上道感染的风险并传播给未感染的伴侣。尽管其基因组非常小（580 kb），但根据与编码免疫优势MgPa粘附素蛋白的 mgpB 基因的同源性，约4％的元素由称为MgPar序列（MgPa重复序列）的重复元素组成。这些MgPar序列的存在以及 M之间的 mgpB 变异性。生殖器菌株表明， mgpB 和MgPar序列重组产生变异的MgPa蛋白。为了检查生物的单个菌株内多样性的程度和产生，我们检查了 M中的 mgpB 变异。生殖器 G-37菌株，观察到的序列异质性可以通过 mgpB 表达位点与推定的供体MgPar序列重组来解释。同样，我们分析了一名持续感染妇女（21个月）的宫颈标本中的 mgpB 序列，并在单个感染 M中鉴定出17种不同的 mgpB 变体。生殖器菌株，证实 mgpB 异质性是在自然感染过程中发生的。这些观察结果支持以下假设： mgpB 基因与MgPar序列发生重组，并且所产生的抗原性不同的MgPa变异体可能有助于免疫逃逸和感染的持续。

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