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Effects of Ploidy and Mating Type on Virulence of Candida albicans

机译:倍性和交配类型对白色念珠菌致病力的影响

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Candida albicans is the most common fungal pathogen of humans. The recent discovery of sexuality in this organism has led to the demonstration of a mating type locus which is usually heterozygous, although some isolates are homozygous. Tetraploids can be formed between homozygotes of the opposite mating type. However, the role of the mating process and tetraploid formation in virulence has not been investigated. We describe here experiments using a murine model of disseminated candidiasis which demonstrate that in three strains, including CAI-4, the most commonly used strain background, tetraploids are less virulent than diploids and can undergo changes in ploidy during infection. In contrast to reports with other strains, we find that MTL homozygotes are almost as virulent as the heterozygotes. These results show that the level of ploidy in Candida albicans can affect virulence, but the mating type configuration does not necessarily do so.
机译:白色念珠菌是人类最常见的真菌病原体。在该生物体中最近发现的性行为已导致证实了一个交配型基因座,该位点通常是杂合的,尽管某些分离株是纯合的。四倍体可以在相反交配类型的纯合子之间形成。但是,尚未研究交配过程和四倍体形成在毒力中的作用。我们在这里描述了使用鼠疫传播的念珠菌病鼠模型进行的实验,该实验表明在三种菌株(包括最常用的菌株背景CAI-4)中,四倍体的毒性低于二倍体,并且在感染过程中会发生倍性变化。与其他菌株的报道相反,我们发现 MTL 纯合子几乎与杂合子一样有毒。这些结果表明,白色念珠菌中的倍性水平可以影响毒力,但交配类型的配置不一定会影响毒力。

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