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首页> 外文期刊>Infection and immunity >Functional Mapping of YadA- and Ail-Mediated Binding of Human Factor H to Yersinia enterocolitica Serotype O:3
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Functional Mapping of YadA- and Ail-Mediated Binding of Human Factor H to Yersinia enterocolitica Serotype O:3

机译：人因子H与小肠结肠炎耶尔森菌血清型O：3的YadA和Ail介导的结合的功能定位。

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Yersinia enterocolitica is an enteric pathogen that exploits diverse means to survive in the human host. Upon Y. enterocolitica entry into the human host, bacteria sense and respond to variety of signals, one of which is the temperature. Temperature in particular has a profound impact on Y. enterocolitica gene expression, as most of its virulence factors are expressed exclusively at 37°C. These include two outer membrane proteins, YadA and Ail, that function as adhesins and complement resistance (CR) factors. Both YadA and Ail bind the functionally active complement alternative pathway regulator factor H (FH). In this study, we characterized regions on both proteins involved in CR and the interaction with FH. Twenty-eight mutants having short (7 to 41 amino acids) internal deletions within the neck and stalk of YadA and two complement-sensitive site-directed Ail mutants were constructed to map the CR and FH binding regions of YadA and Ail. Functional analysis of the YadA mutants revealed that the stalk of YadA is required for both CR and FH binding and that FH appears to target several conformational and discontinuous sites of the YadA stalk. On the other hand, the complement-sensitive Ail mutants were not affected in FH binding. Our results also suggested that Ail- and YadA-mediated CR does not depend solely on FH binding.
机译：肠球菌耶尔森菌是一种肠道病原体，它利用多种手段在人类宿主中生存。在 Y。 Enterocolitica 进入人体宿主后，细菌会感知并响应各种信号，其中之一就是温度。温度尤其对Y产生深远的影响。肠结肠炎基因表达，因为其大多数毒力因子仅在37°C下表达。这些包括两个外膜蛋白YadA和Ail，它们起粘附蛋白和补体抗性（CR）因子的作用。 YadA和Ail都结合功能活性补体旁路途径调节因子H（FH）。在这项研究中，我们表征了参与CR和与FH相互作用的两种蛋白质上的区域。构建了28个YadA的颈和茎内部具有短（7至41个氨基酸）内部缺失的突变体，以及两个补体敏感的定点Ail突变体，以绘制YadA和Ail的CR和FH结合区域的图。 YadA突变体的功能分析显示，CR和FH结合都需要YadA茎，并且FH似乎靶向YadA茎的几个构象和不连续位点。另一方面，对补体敏感的Ail突变体在FH结合中不受影响。我们的研究结果还表明，Ail和YadA介导的CR不仅仅依赖于FH结合。

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