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首页> 外文期刊>Infection and immunity >Protection of Wild-Type and Severe Combined Immunodeficiency Mice against an Intranasal Challenge by Passive Immunization with Monoclonal Antibodies to the Chlamydia trachomatis Mouse Pneumonitis Major Outer Membrane Protein
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Protection of Wild-Type and Severe Combined Immunodeficiency Mice against an Intranasal Challenge by Passive Immunization with Monoclonal Antibodies to the Chlamydia trachomatis Mouse Pneumonitis Major Outer Membrane Protein

机译：通过对沙眼衣原体小鼠肺炎小鼠肺炎主要外膜蛋白的单克隆抗体的被动免疫被动保护野生型和严重的联合免疫缺陷小鼠。

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Monoclonal antibodies (MAbs) to the Chlamydia trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP) were characterized for their ability to neutralize the infectivity of this organism in vitro and in vivo. One of the MAbs (MoPn-23) recognizes a nonlinear epitope in the MOMP, MAb MoPn-40 binds to a linear epitope in the variable domain 1 (VD1), and MAb MoPn-32 recognizes the chlamydial lipopolysaccharide. MAb MoPn-23 neutralized 50% of the infectivity of Chlamydia, as measured in vitro by using HAK (FcγIII?) and HeLa-229 (FcγIII+) cells at a concentration 100 times lower than MAb MoPn-40. MAb MoPn-32 had no neutralizing ability. In comparison to the control normal mouse immunoglobulin G, passive immunization of BALB/c mice with MAb MoPn-23 resulted in a highly significant protection against an intranasal (i.n.) challenge as determined by the change in body weight, the weight of the lungs, and the yield of Chlamydia inclusion-forming units (IFU) from the lungs. Passive immunization with MAb MoPn-40 resulted in a lower degree of protection, and MAb MoPn-32 afforded no protection. MAb MoPn-23 was also tested for its ability to protect wild-type (WT) and severe combined immunodeficient (SCID) C.B-17 mice against an i.n. challenge. Protection based on total body weight, lung weight, and yield of Chlamydia IFU was as effective in SCID as in WT C.B-17 mice. In conclusion, antibodies to MOMP can protect mice against a chlamydial infection in the presence or absence of T and B cells.
机译：针对沙眼衣原体小鼠肺炎（MoPn）主要外膜蛋白（MOMP）的单克隆抗体（MAb）具有中和该生物体在体外和体内感染力的能力。一种单克隆抗体（MoPn-23）识别MOMP中的非线性表位，单克隆抗体MoPn-40结合至可变域1（VD1）中的线性表位，而单克隆抗体MoPn-32识别衣原体脂多糖。通过使用HAK（FcγIII？）和HeLa-229（FcγIII + ）细胞，其浓度比MAb MoPn-40低100倍。单抗MoPn-32没有中和能力。与对照正常小鼠免疫球蛋白G相比，用MAb MoPn-23被动免疫BALB / c小鼠可产生针对鼻内（in）攻击的高度显着保护，这由体重，肺重量，肺中衣原体包涵体形成单位（IFU）的产量。 MAb MoPn-40的被动免疫导致较低的保护程度，而MAb MoPn-32没有提供保护。还测试了单克隆抗体MoPn-23对野生型（WT）和重度联合免疫缺陷（SCID）C.B-17小鼠的抗i.n.挑战。基于总体重，肺重和衣原体IFU产量的保护在SCID中与WT C.B-17小鼠一样有效。总之，在存在或不存在T细胞和B细胞的情况下，针对MOMP的抗体可以保护小鼠免受衣原体感染。

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