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首页> 外文期刊>Infection and immunity >Intracellular Listeria monocytogenes Comprises a Minimal but Vital Fraction of the Intestinal Burden following Foodborne Infection
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Intracellular Listeria monocytogenes Comprises a Minimal but Vital Fraction of the Intestinal Burden following Foodborne Infection

机译：细胞内单核细胞增生李斯特菌包括食源性感染后肠道负担的最小但重要部分

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Listeria monocytogenes is a highly adaptive bacterium that replicates as a free-living saprophyte in the environment as well as a facultative intracellular pathogen that causes invasive foodborne infections. The intracellular life cycle of L. monocytogenes is considered to be its primary virulence determinant during mammalian infection; however, the proportion of L. monocytogenes that is intracellular in vivo has not been studied extensively. In this report, we demonstrate that the majority of wild-type (strain EGDe) and mouse-adapted (InlAm-expressing) L. monocytogenes recovered from the mesenteric lymph nodes (MLN) was extracellular within the first few days after foodborne infection. In addition, significantly lower burdens of L. monocytogenes were recovered from the colon, spleen, and liver of gentamicin-treated mice than of control mice. This led us to investigate whether intracellular replication of L. monocytogenes was essential during the intestinal phase of infection. We found that lipoate protein ligase-deficient L. monocytogenes (ΔlplA1) mutants, which display impaired intracellular growth, were able to colonize the colon but did not persist efficiently and had a significant defect in spreading to the MLN, spleen, and liver. Together, these data indicate that the majority of the L. monocytogenes burden in the gastrointestinal tract is extracellular, but the small proportion of intracellular L. monocytogenes is essential for dissemination to the MLN and systemic organs.
机译：单核细胞增生李斯特菌是一种高度适应性细菌，可在环境中以自由生腐生菌的形式复制，并且是引起侵袭性食源性感染的兼性细胞内病原体。单核细胞增生李斯特菌的细胞内生命周期被认为是哺乳动物感染过程中的主要毒力决定因素。然而，尚未广泛研究体内细胞内单核细胞增生李斯特菌的比例。在本报告中，我们证明从食源性感染后的最初几天内，从肠系膜淋巴结（MLN）回收的大多数野生型（EGDe株）和小鼠适应的（表达InlAm的）单核细胞增生李斯特菌。另外，庆大霉素处理的小鼠从结肠，脾脏和肝脏中回收的单核细胞增生李斯特菌的负担比对照小鼠低得多。这导致我们调查在感染的肠道阶段单核细胞增生李斯特菌的细胞内复制是否必不可少。我们发现，脂蛋白缺乏连接酶的单核细胞增生李斯特氏菌（ΔlplA1）突变体，显示出受损的细胞内生长，能够在结肠中定植，但不能有效地持续存在，并且在传播至MLN，脾脏和肝脏方面存在明显缺陷。总之，这些数据表明，胃肠道中单核细胞增生李斯特菌的大部分负担是细胞外的，但是细胞内单核细胞增生李斯特菌的小比例对于向MLN和全身器官的传播至关重要。

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