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首页> 外文期刊>Infection and immunity >The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model
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The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model

机译：副溶血弧菌ToxRS调节剂是压力耐受性和定植在新型的胃链霉素诱导的成年小鼠模型中所必需的。

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Vibrio parahaemolyticus, a marine bacterium, is the causative agent of gastroenteritis associated with the consumption of seafood. It contains a homologue of the toxRS operon that in V. cholerae is the key regulator of virulence gene expression. We examined a nonpolar mutation in toxRS to determine the role of these genes in V. parahaemolyticus RIMD2210633, an O3:K6 isolate, and showed that compared to the wild type, ΔtoxRS was significantly more sensitive to acid, bile salts, and sodium dodecyl sulfate stresses. We demonstrated that ToxRS is a positive regulator of ompU expression, and that the complementation of ΔtoxRS with ompU restores stress tolerance. Furthermore, we showed that ToxRS also regulates type III secretion system genes in chromosome I via the regulation of the leuO homologue VP0350. We examined the effect of ΔtoxRS in vivo using a new orogastric adult murine model of colonization. We demonstrated that streptomycin-treated adult C57BL/6 mice experienced prolonged intestinal colonization along the entire intestinal tract by the streptomycin-resistant V. parahaemolyticus. In contrast, no colonization occurred in non-streptomycin-treated mice. A competition assay between the ΔtoxRS and wild-type V. parahaemolyticus strains marked with the β-galactosidase gene lacZ demonstrated that the ΔtoxRS strain was defective in colonization compared to the wild-type strain. This defect was rescued by ectopically expressing ompU. Thus, the defect in stress tolerance and colonization in ΔtoxRS is solely due to OmpU. To our knowledge, the orogastric adult murine model reported here is the first showing sustained intestinal colonization by V. parahaemolyticus.
机译：副溶血性弧菌是一种海洋细菌，是与食用海鲜有关的肠胃炎的病原体。它包含toxRS操纵子的同源物，霍乱弧菌是毒力基因表达的关键调节因子。我们检查了toxRS的非极性突变，以确定这些基因在溶血性弧菌RIMD2210633（一种O3：K6分离株）中的作用，并表明与野生型相比，ΔtoxRS对酸，胆汁盐和十二烷基硫酸钠的敏感性更高压力。我们证明了ToxRS是ompU表达的正调节剂，并且与ompU互补的ΔtoxRS恢复了压力耐受性。此外，我们显示ToxRS还通过leuO同源物VP0350的调控来调控I号染色体中的III型分泌系统基因。我们使用新的口胃成年鼠定殖模型检查了ΔtoxRS在体内的作用。我们证明了链霉素治疗的成年C57BL / 6小鼠经历了链霉素抗性副溶血弧菌在整个肠道的延长肠道定殖。相反，在未用链霉素治疗的小鼠中没有定植。在ΔtoxRS与标记有β-半乳糖苷酶基因lacZ的野生型副溶血弧菌之间的竞争试验表明，与野生型菌株相比，ΔtoxRS菌株在定殖中有缺陷。通过异位表达ompU可以挽救该缺陷。因此，ΔtoxRS中的耐应力性和定植性缺陷仅归因于OmpU。据我们所知，这里报道的口胃成年鼠模型是第一个显示副溶血弧菌持续肠道定植的模型。

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