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首页> 外文期刊>Infection and immunity >Contribution of Siderophore Systems to Growth and Urinary Tract Colonization of Asymptomatic Bacteriuria Escherichia coli
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Contribution of Siderophore Systems to Growth and Urinary Tract Colonization of Asymptomatic Bacteriuria Escherichia coli

机译：铁载体系统对无症状细菌性大肠杆菌的生长和尿道定植的贡献

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The molecular mechanisms that define asymptomatic bacteriuria (ABU) Escherichia coli colonization of the human urinary tract remain to be properly elucidated. Here, we utilize ABU E. coli strain 83972 as a model to dissect the contribution of siderophores to iron acquisition, growth, fitness, and colonization of the urinary tract. We show that E. coli 83972 produces enterobactin, salmochelin, aerobactin, and yersiniabactin and examine the role of these systems using mutants defective in siderophore biosynthesis and uptake. Enterobactin and aerobactin contributed most to total siderophore activity and growth in defined iron-deficient medium. No siderophores were detected in an 83972 quadruple mutant deficient in all four siderophore biosynthesis pathways; this mutant did not grow in defined iron-deficient medium but grew in iron-limited pooled human urine due to iron uptake via the FecA ferric citrate receptor. In a mixed 1:1 growth assay with strain 83972, there was no fitness disadvantage of the 83972 quadruple biosynthetic mutant, demonstrating its capacity to act as a “cheater” and utilize siderophores produced by the wild-type strain for iron uptake. An 83972 enterobactin/salmochelin double receptor mutant was outcompeted by 83972 in human urine and the mouse urinary tract, indicating a role for catecholate receptors in urinary tract colonization.
机译：定义无症状性细菌尿症（ABU）人尿道的大肠杆菌定殖的分子机制仍有待正确阐明。在这里，我们利用ABU大肠杆菌菌株83972作为模型来分析铁载体对铁的获取，生长，适应性和尿道定植的贡献。我们显示大肠杆菌83972产生肠抑菌素，沙门氏菌素，气杆菌素和耶尔西菌素，并使用在铁载体生物合成和摄取中有缺陷的突变体检查这些系统的作用。在限定的缺铁培养基中，肠杆菌素和航空杆菌素对总铁载体活性和生长的贡献最大。在所有四个铁载体生物合成途径均缺失的83972个四倍体突变体中未检测到铁载体。由于通过FecA柠檬酸铁受体吸收铁，该突变体没有在特定的缺铁培养基中生长，但是在有限铁的人类尿液中生长。在与83972菌株进行的1：1混合生长试验中，没有83839四倍生物合成突变体的适应性劣势，证明了其充当“骗子”并利用野生型菌株产生的铁载体吸收铁的能力。 83972肠杆菌素/沙门氏菌素双受体突变体在人尿和小鼠尿道中被83972竞争，表明儿茶酚酸受体在尿道定植中的作用。

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