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首页> 外文期刊>Infection and immunity >pncA and bptA Are Not Sufficient To Complement Ixodes scapularis Colonization and Persistence by Borrelia burgdorferi in a Linear Plasmid lp25-Deficient Background
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pncA and bptA Are Not Sufficient To Complement Ixodes scapularis Colonization and Persistence by Borrelia burgdorferi in a Linear Plasmid lp25-Deficient Background

机译：pncA和bptA在线性质粒lp25缺乏的背景下不足以补充肩x小球藻的定殖和持久性。

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The complex segmented genome of Borrelia burgdorferi is comprised of a linear chromosome along with numerous linear and circular plasmids essential for tick and/or mammalian infectivity. The pathogenic necessity for specific borrelial plasmids has been identified; most notably, infections of the tick vector and mammalian host both require linear plasmid 25 (lp25). Genes carried on lp25, specifically bptA and pncA, are postulated to play a role for B. burgdorferi to infect and persist in Ixodes ticks. In this study, we complemented an lp25-deficient borrelial strain with pncA alone or pncA accompanied by bptA to evaluate the ability of the complemented strains to restore larval colonization and persistence through transstadial transmission relative to that of wild-type B. burgdorferi. The acquisition of the complemented strains by tick larvae from infected mice and/or the survival of these strains was significantly decreased when assayed by cultivation and quantitative PCR (qPCR). Only 10% of the pncA-complemented strain organisms were found by culture to survive 17 days following larval feeding, while 45% of the pncA- and bptA-complemented strain organisms survived, with similar results by PCR. However, neither of the complemented B. burgdorferi strains was capable of persisting through the molt to the nymphal stage as analyzed by culture. qPCR analyses of unfed nymphs detected B. burgdorferi genomes in several nymphs at low copy numbers, likely indicating the presence of DNA from dead or dying cells. Overall, the data indicate that pncA and bptA cannot independently support infection, suggesting that lp25 carries additional gene(s) or regulatory elements critical for B. burgdorferi survival and pathogenesis in the Ixodes vector.
机译：伯氏疏螺旋体的复杂分段基因组由线性染色体以及对tick和/或哺乳动物感染性必不可少的许多线性和环状质粒组成。已经确定了特定硼质质粒的致病必要性。最值得注意的是，壁虱载体和哺乳动物宿主的感染都需要线性质粒25（lp25）。假定在lp25上携带的基因，特别是bptA和pncA，被认为对伯氏疏螺旋体具有感染作用并持续存在于x虱中。在这项研究中，我们用单独的pncA或pncA加上bptA补充了lp25缺陷型波尔氏菌株，以评估与野生型B. burgdorferi相比，补充菌株通过跨虫传播恢复幼虫定殖和持久性的能力。通过培养和定量PCR（qPCR）进行分析时，tick虫幼虫从感染小鼠中获取补充株和/或这些株的存活率显着降低。通过培养发现，只有10％的pncA补充菌株有机体在喂食幼虫后可以存活17天，而pncA和bptA补充的菌株有机体则存活了45％，PCR结果相似。然而，如通过培养分析的，互补的伯氏疏螺旋体菌株均不能通过蜕皮持续到若虫期。对未进食若虫的qPCR分析检测到了数只若虫中B. burgdorferi基因组，它们的拷贝数很低，可能表明死细胞或垂死细胞中存在DNA。总体而言，数据表明pncA和bptA不能独立支持感染，这表明lp25在Ixodes载体中携带了对B. burgdorferi生存和发病机理至关重要的其他基因或调控元件。

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