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Porphyromonas gingivalis-Host Interactions in a Drosophila melanogaster Model

机译：果蝇黑色素瘤模型中的牙龈卟啉单胞菌-宿主相互作用

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Porphyromonas gingivalis is a Gram-negative obligate anaerobe that has been implicated in the etiology of adult periodontitis. We recently introduced a Drosophila melanogaster killing model for examination of P. gingivalis-host interactions. In the current study, the Drosophila killing model was used to characterize the host response to P. gingivalis infection by identifying host components that play a role during infection. Drosophila immune response gene mutants were screened for altered susceptibility to killing by P. gingivalis. The Imd signaling pathway was shown to be important for the survival of Drosophila infected by nonencapsulated P. gingivalis strains but was dispensable for the survival of Drosophila infected by encapsulated P. gingivalis strains. The P. gingivalis capsule was shown to mediate resistance to killing by Drosophila antimicrobial peptides (Imd pathway-regulated cecropinA and drosocin) and human beta-defensin 3. Drosophila thiol-ester protein II (Tep II) and Tep IV and the tumor necrosis factor (TNF) homolog Eiger were also involved in the immune response against P. gingivalis infection, while the scavenger receptors Eater and Croquemort played no roles in the response to P. gingivalis infection. This study demonstrates that the Drosophila killing model is a useful high-throughput model for characterizing the host response to P. gingivalis infection and uncovering novel interactions between the bacterium and the host.
机译：牙龈卟啉单胞菌是革兰氏阴性专性厌氧菌，与成人牙周炎的病因有关。最近，我们引入了果蝇杀死果蝇的模型，用于研究牙龈卟啉单胞菌与宿主之间的相互作用。在当前的研究中，果蝇杀死模型用于通过鉴定在感染过程中起作用的宿主组分来表征宿主对牙龈卟啉单胞菌感染的反应。筛选果蝇免疫应答基因突变体对牙龈卟啉单胞菌杀死的敏感性的改变。已显示，Imd信号通路对于非包膜牙龈卟啉单胞菌菌株感染的果蝇的存活很重要，但对于被包囊牙龈丙酸杆菌菌株感染的果蝇的存活是必不可少的。牙龈卟啉单胞菌胶囊显示通过果蝇抗菌肽（IMd途径调节的crocropinA和drosocin）和人β-防御素3介导对杀伤的抗性。果蝇硫醇酯蛋白II（Tep II）和Tep IV和肿瘤坏死因子（TNF）同系物Eiger也参与了针对牙龈卟啉单胞菌感染的免疫反应，而清道夫受体Eater和Croquemort在对牙龈卟啉单胞菌感染的应答中不起作用。这项研究表明果蝇杀死模型是有用的高通量模型，用于表征宿主对牙龈卟啉单胞菌感染的反应并揭示细菌与宿主之间的新型相互作用。

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