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首页> 外文期刊>Infection and immunity >Expression of STM4467-Encoded Arginine Deiminase Controlled by the STM4463 Regulator Contributes to Salmonella enterica Serovar Typhimurium Virulence
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Expression of STM4467-Encoded Arginine Deiminase Controlled by the STM4463 Regulator Contributes to Salmonella enterica Serovar Typhimurium Virulence

机译：由STM4463调节剂控制的STM4467编码的精氨酸脱亚氨酶的表达有助于肠炎沙门氏菌血清鼠伤寒毒力。

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摘要

Arginine deiminase (ADI), carbamate kinase (CK), and ornithine transcarbamoylase (OTC) constitute the ADI system. In addition to metabolic functions, the ADI system has been implicated in the virulence of certain pathogens. The pathogenic intracellular bacterium Salmonella enterica serovar Typhimurium possesses the STM4467, STM4466, and STM4465 genes, which are predicted to encode ADI, CK, and OTC, respectively. Here we report that the STM4467 gene encodes an ADI and that ADI activity plays a role in the successful infection of a mammalian host by S. Typhimurium. An STM4467 deletion mutant was defective for replication inside murine macrophages and was attenuated for virulence in mice. We determined that a regulatory protein encoded by the STM4463 gene functions as an activator for STM4467 expression. The expression of the ADI pathway genes was enhanced inside macrophages in a process that required STM4463. Lack of STM4463 impaired the ability of S. Typhimurium to replicate within macrophages. A mutant defective in STM4467-encoded ADI displayed normal production of nitric oxide by macrophages.
机译：精氨酸脱亚氨酶（ADI），氨基甲酸酯激酶（CK）和鸟氨酸转氨甲酰酶（OTC）构成ADI系统。除代谢功能外，ADI系统还涉及某些病原体的毒力。病原性细胞内肠炎沙门氏菌鼠伤寒沙门氏菌具有 STM4467 ， STM4466 和 STM4465 基因，预计可编码ADI，CK和OTC ， 分别。在这里，我们报道 STM4467 基因编码一个ADI，而ADI活性在 S 成功感染哺乳动物宿主中发挥作用。鼠伤寒一个 STM4467 缺失突变体在鼠巨噬细胞内的复制有缺陷，而在小鼠中的毒力却减弱了。我们确定 STM4463 基因编码的调节蛋白起 STM4467 表达激活剂的作用。在需要STM4463的过程中，巨噬细胞内部ADI途径基因的表达得以增强。缺少STM4463会损害 S 的功能。鼠伤寒在巨噬细胞内复制。在 STM4467 编码的ADI中有缺陷的突变体显示巨噬细胞正常产生一氧化氮。

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