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首页> 外文期刊>Infection and immunity >Role of Pht Proteins in Attachment of Streptococcus pneumoniae to Respiratory Epithelial Cells
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Role of Pht Proteins in Attachment of Streptococcus pneumoniae to Respiratory Epithelial Cells

机译：Pht蛋白在肺炎链球菌附着于呼吸道上皮细胞中的作用

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Pneumococcal adherence to mucosal surfaces is a critical step in nasopharyngeal colonization, but so far few pneumococcal adhesins involved in the interaction with host cells have been identified. PhtA, PhtB, PhtD, and PhtE are conserved pneumococcal surface proteins that have proven promising as vaccine candidates. One suggested virulence function of Pht proteins is to mediate adherence at the respiratory mucosa. In this study, we assessed the role of Pht proteins in pneumococcal binding to respiratory epithelial cells. Pneumococci were incubated with human nasopharyngeal epithelial cells (Detroit-562) and lung epithelial cells (A549 and NCI-H292), and the proportion of bound bacteria was measured by plating viable counts. Strains R36A (unencapsulated), D39 (serotype 2), 43 (serotype 3), 4-CDC (serotype 4), and 2737 (serotype 19F) with one or more of the four homologous Pht proteins deleted were compared with their wild-type counterparts. Also, the effect of anti-PhtD antibodies on the adherence of strain 2737 to the respiratory epithelial cells was studied. Our results suggest that Pht proteins play a role in pneumococcal adhesion to the respiratory epithelium. We also found that antibody to PhtD is able to inhibit bacterial attachment to the cells, suggesting that antibodies against PhtD present at mucosal surfaces might protect from pneumococcal attachment and subsequent colonization. However, the relative significance of Pht proteins to the ability of pneumococci to bind in vitro to epithelial cells depends on the genetic background and the capsular serotype of the strain.
机译：肺炎球菌对粘膜表面的粘附是鼻咽菌落定殖的关键步骤，但是到目前为止，几乎没有发现与宿主细胞相互作用的肺炎球菌粘附素。 PhtA，PhtB，PhtD和PhtE是保守的肺炎球菌表面蛋白，已被证明可作为疫苗候选物。 Pht蛋白的一种毒力功能是介导呼吸道粘膜的粘附。在这项研究中，我们评估了Pht蛋白在肺炎球菌与呼吸道上皮细胞结合中的作用。将肺炎球菌与人鼻咽上皮细胞（底特律562）和肺上皮细胞（A549和NCI-H292）一起孵育，并通过接种活计数来测定结合细菌的比例。将删除了四个同源Pht蛋白中的一种或多种的R36A（未封装），D39（血清型2），43（血清型3），4-CDC（血清型4）和2737（血清型19F）菌株与野生型进行了比较。同行。此外，还研究了抗PhtD抗体对2737菌株与呼吸道上皮细胞粘附的影响。我们的结果表明Pht蛋白在肺炎球菌对呼吸道上皮的粘附中起作用。我们还发现针对PhtD的抗体能够抑制细菌附着至细胞，这表明针对存在于粘膜表面的PhtD抗体可能会保护免受肺炎球菌附着和随后的定居。然而，Pht蛋白对肺炎球菌在体外与上皮细胞结合的能力的相对重要性取决于菌株的遗传背景和荚膜血清型。

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