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首页> 外文期刊>Infection and immunity >An Iron-Regulated Autolysin Remodels the Cell Wall To Facilitate Heme Acquisition in Staphylococcus lugdunensis
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An Iron-Regulated Autolysin Remodels the Cell Wall To Facilitate Heme Acquisition in Staphylococcus lugdunensis

机译：铁调节的自溶素重塑了细胞壁，以促进血球菌lugdunensis血红素的获取。

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Bacteria alter their cell surface in response to changing environments, including those encountered upon invasion of a host during infection. One alteration that occurs in several Gram-positive pathogens is the presentation of cell wall-anchored components of the iron-regulated surface determinant (Isd) system, which extracts heme from host hemoglobin to fulfill the bacterial requirement for iron. Staphylococcus lugdunensis, an opportunistic pathogen that causes infective endocarditis, encodes an Isd system. Unique among the known Isd systems, S. lugdunensis contains a gene encoding a putative autolysin located adjacent to the Isd operon. To elucidate the function of this putative autolysin, here named IsdP, we investigated its contribution to Isd protein localization and hemoglobin-dependent iron acquisition. S. lugdunensis IsdP was found to be iron regulated and cotranscribed with the Isd operon. IsdP is a specialized peptidoglycan hydrolase that cleaves the stem peptide and pentaglycine crossbridge of the cell wall and alters processing and anchoring of a major Isd system component, IsdC. Perturbation of IsdC localization due to isdP inactivation results in a hemoglobin utilization growth defect. These studies establish IsdP as an autolysin that functions in heme acquisition and describe a role for IsdP in cell wall reorganization to accommodate nutrient uptake systems during infection.
机译：细菌会响应不断变化的环境而改变其细胞表面，包括在感染过程中入侵宿主时所遇到的环境。在几种革兰氏阳性病原体中发生的一种变化是铁调节表面决定簇（Isd）系统的细胞壁锚定成分的呈现，该系统从宿主血红蛋白中提取血红素来满足细菌对铁的需求。导致传染性心内膜炎的机会病原体葡萄球菌葡萄球菌编码Isd系统。在已知的Isd系统中独一无二的是，卢格敦链球菌（S. lugdunensis）包含一个编码与Isd操纵子相邻的假定自溶素的基因。为了阐明这种假定的自溶素（此处称为IsdP）的功能，我们研究了其对Isd蛋白定位和血红蛋白依赖性铁获得的贡献。发现卢格敦链球菌IsdP受铁调节并与Isd操纵子共转录。 IsdP是一种专门的肽聚糖水解酶，可切割细胞壁的干肽和五甘氨酸交叉桥，并改变主要Isd系统组分IsdC的加工和锚定。由于isdP失活而引起的IsdC定位扰动会导致血红蛋白利用增长缺陷。这些研究将IsdP确立为在血红素获取中发挥功能的自溶素，并描述了IsdP在细胞壁重组中的作用，以适应感染过程中的营养吸收系统。

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