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首页> 外文期刊>International journal of infectious diseases : >Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS)
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Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS)

机译：存活严重血小板减少综合征（SFTS）患者的T淋巴细胞免疫学特征的动态变化

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Objective Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with high mortality. T cell deficiency has recently been described, but the changes in T cell functionality during acute SFTS virus (SFTSV) infection and the mechanisms leading to T lymphocyte death remain largely unknown. This study was conducted to evaluate T cell functionality and the expression of apoptotic/proliferation and activation/inhibition markers during acute SFTSV infection. Methods Twenty-eight surviving SFTS patients were sequentially sampled during their entire hospital stay. SFTSV RNA copies were investigated using real-time RT-PCR. The expression levels of apoptotic markers (annexin V and CD95) and proliferation and activation markers (Ki-67, HLA-DR, and CD25) and the expression levels of programmed cell death-1 (PD-1), interferon gamma (IFN-γ), and granzyme B in T cells were evaluated by flow cytometry for the SFTS patients. Results In parallel with T cell depletion, higher annexin V and CD95 expression was observed in SFTS patients. Additionally, the expression levels of Ki-67, HLA-DR, CD25, and PD-1 and the levels of IFN-γ and granzyme B in T lymphocytes were markedly increased in the SFTS patients. Conclusions T cell proliferation, activation, and functional enhancement were apparent despite the observation of T cell apoptosis, suggesting that these processes are involved in the complex protective response to SFTSV infection.
机译：目的严重发热伴血小板减少综合征（SFTS）是一种高死亡率的新兴传染病。最近已经描述了T细胞缺乏症，但是在急性SFTS病毒（SFTSV）感染期间T细胞功能的变化以及导致T淋巴细胞死亡的机制仍然未知。进行这项研究以评估急性SFTSV感染期间T细胞功能以及凋亡/增殖和活化/抑制标志物的表达。方法在整个住院期间，对28名幸存的SFTS患者进行了顺序采样。使用实时RT-PCR研究了SFTSV RNA拷贝。凋亡标记物（annexin V和CD95），增殖和激活标记物（Ki-67，HLA-DR和CD25）的表达水平以及程序性细胞死亡1（PD-1），γ干扰素（IFN-γ）的表达水平。通过流式细胞术评估了SFTS患者的T细胞中的γ）和颗粒酶B。结果与T细胞耗竭同时，在SFTS患者中观察到更高的膜联蛋白V和CD95表达。此外，SFTS患者中T淋巴细胞中Ki-67，HLA-DR，CD25和PD-1的表达水平以及IFN-γ和颗粒酶B的水平显着增加。结论尽管观察到T细胞凋亡，但T细胞的增殖，活化和功能增强仍然很明显，表明这些过程参与了对SFTSV感染的复杂保护反应。

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