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Bacterial pathogen spectrum of acute diarrheal outpatients in an urbanized rural district in Southwest China

机译:西南城市化农村地区急性腹泻患者的细菌病原体谱

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Objectives To conduct a one-year pathogen surveillance of acute diarrheal disease based on outpatient clinics in township hospitals in rural Hongta District of Yunnan Province, China. Methods Fecal specimens of acute diarrhea cases and relevant epidemiological information were collected. Salmonella, Shigella, Vibrio, Aeromonas, Plesiomonas shigelloides and diarrheogenic Escherichia coli (DEC) were examined. Results Among the 797 stool specimens sampled, 198 samples (24.8%) were positive in pathogen isolation, and 223 strains were isolated. The order of isolation rates from high to low were DEC , Aeromonas, P. shigelloides, Salmonella, Shigella and Vibrio . The overall positive rate in middle school students and preschool children was relatively high; while the overall positive rate of less than 1-year-old infants and above 55 years olds was relatively low. The isolates were analyzed by pulsed-field gel electrophoresis (PFGE). Some cases had the same or very close onset time, and the isolates had similar PFGE patterns, suggesting a possible outbreak once occurred but was not detected by the current infectious disease reporting system. Conclusions Pathogen infection and transmission in rapidly urbanized rural areas is a serious issue. There is a great need for a more sensitive and accurate mode of monitoring, reporting and outbreak identification of diarrheal disease.
机译:目的在云南省红塔县农村乡镇卫生院门诊进行为期一年的急性腹泻病病原体监测。方法收集急性腹泻患者粪便标本及相关流行病学资料。检查了沙门氏菌,志贺氏菌,弧菌,气单胞菌,志贺氏假单胞菌和腹泻性大肠杆菌(DEC)。结果在797份粪便标本中,有198份(24.8%)的病原菌分离阳性,分离出223株。隔离率从高到低的顺序依次为DEC,气单胞菌,志贺氏疟原虫,沙门氏菌,志贺氏菌和弧菌。中学生和学龄前儿童的总体阳性率较高; 1岁以下婴儿和55岁以上婴儿的总体阳性率相对较低。通过脉冲场凝胶电泳(PFGE)分析分离物。一些病例的发作时间相同或非常接近,而且分离株的PFGE模式相似,这表明一旦发生可能就可能爆发,但当前的传染病报告系统并未发现。结论快速城市化农村地区的病原体感染和传播是一个严重的问题。迫切需要一种更灵敏,准确的监测,报告和腹泻病暴发识别的模式。

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