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首页> 外文期刊>International Journal of Molecular Sciences >Cloning and Expression Analysis of a PISTILLATA Homologous Gene from Pineapple (Ananas comosus L. Merr)
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Cloning and Expression Analysis of a PISTILLATA Homologous Gene from Pineapple (Ananas comosus L. Merr)

机译：菠萝（Ananas comosus L. Merr）的PISTILLATA同源基因的克隆和表达分析

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PISTILLATA (PI)-like genes are crucial regulators of flowering in angiosperms. A homologue of PI, designated as AcPI (Genbank accession number HQ717796), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcPI is 907 bp in length and contains an open reading frame of 594 bp, which encodes a protein of 197 amino acids. The molecular weight was 2.29 kDa and the isoelectric point was 9.28. The alignment showed that AcPI had a high identity with CsPIC2 (78.6%), AoPI (77.4%), OrcPI (75.7%) and HPI2 (72.4%). Quantitative real-time polymerase chain reaction (qRT-PCR) analyses in different tissues showed that the expression pattern of AcPI was different from the B-class genes in eudicots. AcPI was expressed in all the tissues investigated. The expression level was very low in fruit stems, bracts, leaves and sepals, high in petals and carpels, and moderate in apical meristems, flesh and stamens. The qRT-PCR analyses in different stages indicated that the expression of AcPI reached the highest level at 40 days after flower inducement, when the multiple fruit and floral organs were forming. It proved the important role of AcPI in floral organs and fruit development. The 35S::AcPI transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.
机译：像PISTILLATA（PI）一样的基因是被子植物开花的重要调节剂。通过逆转录酶聚合酶链反应（RT-PCR）和cDNA末端的快速扩增（RACE）从菠萝品种Comte de Paris分离出PI的同系物，命名为AcPI（Genbank登录号HQ717796）。 AcPI的cDNA序列长度为907 bp，并包含一个594 bp的开放阅读框，其编码197个氨基酸的蛋白质。分子量为2.29kDa，等电点为9.28。比对表明，AcPI与CsPIC2（78.6％），AoPI（77.4％），OrcPI（75.7％）和HPI2（72.4％）具有高度同一性。实时定量聚合酶链反应（qRT-PCR）分析在不同组织中显示，AcPI的表达模式不同于双子叶植物中的B类基因。 AcPI在所有研究的组织中表达。在果茎，片，叶子和萼片中的表达水平非常低，在花瓣和心皮中的表达水平非常高，而在分生组织，果肉和雄蕊中的表达水平中等。在不同阶段的qRT-PCR分析表明，当多个果实和花器官形成时，花诱导后第40天AcPI的表达达到最高水平。它证明了AcPI在花卉器官和果实发育中的重要作用。 35S :: AcPI转基因拟南芥植物比野生型植物更早开花，有更多的花序或分支。

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