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首页> 外文期刊>International Journal of Molecular Sciences >Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis
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Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis

机译：新型水杨酸类似物在拟南芥中诱导强效防御反应。

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The master regulator of salicylic acid (SA)-mediated plant defense, NPR1 (NONEXPRESSER OF PR GENES 1) and its paralogs NPR3 and NPR4, act as SA receptors. After the perception of a pathogen, plant cells produce SA in the chloroplast. In the presence of SA, NPR1 protein is reduced from oligomers to monomers, and translocated into the nucleus. There, NPR1 binds to TGA, TCP, and WRKY transcription factors to induce expression of plant defense genes. A list of compounds structurally similar to SA was generated using ChemMine Tools and its Clustering Toolbox. Several of these analogs can induce SA-mediated defense and inhibit growth of Pseudomonas syringae in Arabidopsis . These analogs, when sprayed on Arabidopsis , can induce the accumulation of the master regulator of plant defense NPR1. In a yeast two-hybrid system, these analogs can strengthen the interactions among NPR proteins. We demonstrated that these analogs can induce the expression of the defense marker gene PR1 . Furthermore, we hypothesized that these SA analogs could be potent tools against the citrus greening pathogen Candidatus liberibacter spp. In fact, our results suggest that the SA analogs we tested using Arabidopsis may also be effective for inducing a defense response in citrus. Several SA analogs consistently strengthened the interactions between citrus NPR1 and NPR3 proteins in a yeast two-hybrid system. In future assays, we plan to test whether these analogs avoid degradation by SA hydroxylases from plant pathogens. In future assays, we plan to test whether these analogs avoid degradation by SA hydroxylases from plant pathogens.
机译：水杨酸（SA）介导的植物防御的主要调节剂NPR1（PR基因的非表达者1）及其旁系同源物NPR3和NPR4充当SA受体。感知病原体后，植物细胞在叶绿体中产生SA。在SA的存在下，NPR1蛋白从寡聚体还原为单体，并转移到细胞核中。在那里，NPR1与TGA，TCP和WRKY转录因子结合，以诱导植物防御基因的表达。使用ChemMine工具及其“聚类工具箱”生成了一系列与SA结构相似的化合物。这些类似物中的几种可诱导拟南芥中SA介导的防御并抑制丁香假单胞菌的生长。这些类似物喷洒在拟南芥上时，可诱导植物防御NPR1主调节剂的积累。在酵母双杂交系统中，这些类似物可以增强NPR蛋白之间的相互作用。我们证明了这些类似物可以诱导防御标记基因PR1的表达。此外，我们假设这些SA类似物可能是对抗柑橘绿化病原体假丝酵母Candidatus liberibacter spp的有效工具。实际上，我们的结果表明我们使用拟南芥测试的SA类似物也可能有效诱导柑橘的防御反应。几个SA类似物在酵母双杂交系统中不断增强柑橘NPR1和NPR3蛋白之间的相互作用。在未来的分析中，我们计划测试这些类似物是否避免植物病原体的SA羟化酶降解。在未来的分析中，我们计划测试这些类似物是否避免植物病原体的SA羟化酶降解。

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