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首页> 外文期刊>International journal of infectious diseases : >NDM-1-producing Enterobacteriaceae in a teaching hospital in Shanghai, China: IncX3-type plasmids may contribute to the dissemination of bla'N'D'M'-'1
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NDM-1-producing Enterobacteriaceae in a teaching hospital in Shanghai, China: IncX3-type plasmids may contribute to the dissemination of bla'N'D'M'-'1

机译：中国上海一家教学医院的产NDM-1肠杆菌科：IncX3型质粒可能有助于bla“ N” D“ M”-“ 1的传播

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Objectives: To provide the epidemiological dissemination and the genetic characteristics of bla"N"D"M"-"1 in a teaching hospital in Shanghai, China. Methods: Here, the carbapenemase genes of 114 CRE isolates were evaluated by polymerase chain reaction (PCR). Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE). Conjugation experiments and Southern blot hybridization were performed to determine the transferability of plasmids. Then plasmids were completely sequenced by the shotgun method. Results: Two Klebsiella pneumoniae strains (RJA1227 and RJF866) and one Raoultella planticola strain (RJA274) were identified as NDM-1 positive. The two K. pneumoniae isolates belonged to ST11 and exhibited highly similar PFGE patterns. Shotgun sequencing showed that plasmid pRJF866 (ca. 110kb) contained genes associated with the IncFII-FIB group and was highly similar to plasmid pKOX
机译：目的：在上海的一家教学医院中提供bla“ N” D“ M”-“ 1的流行病学传播和遗传特征。方法：在这里，通过聚合酶链反应（PCR）评估了114个CRE菌株的碳青霉烯酶基因（ PCR）。通过脉冲场凝胶电泳（PFGE）评估克隆相关性，进行缀合实验和Southern blot杂交以确定质粒的可转移性，然后使用the弹枪法对质粒进行完整测序。结果：2株肺炎克雷伯菌（RJA1227） （RJF866和RJF866）和一株拉乌尔氏菌（Raoultella planticola）菌株（RJA274）被鉴定为NDM-1阳性，这两个肺炎克雷伯菌分离株属于ST11，表现出高度相似的PFGE模式; Shot弹枪测序表明，质粒pRJF866（约110kb）含有与IncFII-FIB组，与质粒pKOX高度相似

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