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首页> 外文期刊>International Journal of Molecular Sciences >Expression Patterns, Activities and Carbohydrate-Metabolizing Regulation of Sucrose Phosphate Synthase, Sucrose Synthase and Neutral Invertase in Pineapple Fruit during Development and Ripening
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Expression Patterns, Activities and Carbohydrate-Metabolizing Regulation of Sucrose Phosphate Synthase, Sucrose Synthase and Neutral Invertase in Pineapple Fruit during Development and Ripening

机译：菠萝果实发育和成熟过程中蔗糖磷酸合酶，蔗糖合酶和中性转化酶的表达模式，活性和糖代谢调控

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Differences in carbohydrate contents and metabolizing-enzyme activities were monitored in apical, medial, basal and core sections of pineapple (Ananas comosus cv. Comte de paris) during fruit development and ripening. Fructose and glucose of various sections in nearly equal amounts were the predominant sugars in the fruitlets, and had obvious differences until the fruit matured. The large rise of sucrose/hexose was accompanied by dramatic changes in sucrose phosphate synthase (SPS) and sucrose synthase (SuSy) activities. By contrast, neutral invertase (NI) activity may provide a mechanism to increase fruit sink strength by increasing hexose concentrations. Furthermore, two cDNAs of Ac-sps (accession no. GQ996582) and Ac-ni (accession no. GQ996581) were first isolated from pineapple fruits utilizing conserved amino-acid sequences. Homology alignment reveals that the amino acid sequences contain some conserved function domains. Transcription expression analysis of Ac-sps, Ac-susy and Ac-ni also indicated distinct patterns related to sugar accumulation and composition of pineapple fruits. It suggests that differential expressions of multiple gene families are necessary for sugar metabolism in various parts and developmental stages of pineapple fruit. A cycle of sucrose breakdown in the cytosol of sink tissues could be mediated through both Ac-SuSy and Ac-NI, and Ac-NI could be involved in regulating crucial steps by generating sugar signals to the cells in a temporally and spatially restricted fashion.
机译：在果实发育和成熟过程中，对菠萝（Ananas comosus cv。Comte de paris）的顶端，内侧，基础和核心部分的碳水化合物含量和代谢酶活性进行了监测。果实中不同部位的果糖和葡萄糖含量几乎相等，是果实中的主要糖分，直到果实成熟为止，两者之间存在明显的差异。蔗糖/己糖的大量增加伴随着蔗糖磷酸合成酶（SPS）和蔗糖合成酶（SuSy）活性的急剧变化。相比之下，中性转化酶（NI）活性可能提供了通过增加己糖浓度来增加果实吸收强度的机制。此外，首先利用保守的氨基酸序列从菠萝果实中分离出Ac-sps（登录号GQ996582）和Ac-ni（登录号GQ996581）的两个cDNA。同源性比对揭示氨基酸序列包含一些保守的功能域。 Ac-sps，Ac-susy和Ac-ni的转录表达分析也表明与菠萝果实糖分积累和组成有关的独特模式。这表明菠萝果实各个部位和发育阶段糖代谢需要多个基因家族的差异表达。可以通过Ac-SuSy和Ac-NI介导蔗糖组织胞浆中蔗糖分解的周期，并且Ac-NI可以通过以时间和空间限制的方式向细胞产生糖信号来参与调节关键步骤。

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