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首页> 外文期刊>International Journal of Molecular Sciences >Chikusetsu Saponin V Attenuates MPP+-Induced Neurotoxicity in SH-SY5Y Cells via Regulation of Sirt1/Mn-SOD and GRP78/Caspase-12 Pathways
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Chikusetsu Saponin V Attenuates MPP+-Induced Neurotoxicity in SH-SY5Y Cells via Regulation of Sirt1/Mn-SOD and GRP78/Caspase-12 Pathways

机译：Chikusetsu Saponin V通过调节Sirt1 / Mn-SOD和GRP78 / Caspase-12途径减轻MPP + 诱导的SH-SY5Y细胞神经毒性。

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Studies have shown that saponins from Panax japonicus (SPJ) possess neuroprotective effects. However, whether Chikusetsu saponin V (CsV), the most abundant member of SPJ, can exert neuroprotective effects against 1-methyl-4-phenylpyridinium ion (MPP+)-induced cytotoxicity is not known. In this study, we aimed to investigate the neuroprotective effects of CsV on MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells and explore its possible mechanisms. Our results show that CsV attenuates MPP+-induced cytotoxicity, inhibits ROS accumulation, and increases mitochondrial membrane potential dose-dependently. We also found that levels of Sirt1 protein and Mn-SOD mRNA significantly decreased in MPP+-treated group but were restored with CsV treatment in a dose-dependent manner. Furthermore, GRP78 protein and Caspase-12 mRNA levels were elevated by MPP+ exposure but reversed by CsV treatment. CsV inhibited the MPP+-induced downregulation of Bcl-2 and up-regulation of Bax in a dose-dependent manner and, thus, increased the ratio of Bcl-2/Bax. Overall, these results suggest that Sirt1/Mn-SOD and GRP78/Caspase-12 pathways might be involved in the CsV-mediated neuroprotective effects.
机译：研究表明，人参中的皂苷具有神经保护作用。但是，尚不清楚SPJ中最丰富的成员Chikusetsu saponin V（CsV）是否能对1-甲基-4-苯基吡啶鎓离子（MPP + ）诱导的细胞毒性发挥神经保护作用。本研究旨在探讨CsV对人成神经细胞瘤SH-SY5Y细胞中MPP + 诱导的细胞毒性的神经保护作用，并探讨其可能的机制。我们的结果表明，CsV减弱了MPP + 诱导的细胞毒性，抑制了ROS的积累，并剂量依赖性地增加了线粒体膜电位。我们还发现，MPP + 治疗组的Sirt1蛋白和Mn-SOD mRNA水平显着降低，但CsV治疗后剂量依赖性恢复。此外，MPP + 暴露使GRP78蛋白和Caspase-12 mRNA水平升高，而经CsV处理则逆转。 CsV以剂量依赖的方式抑制了MPP + 诱导的Bcl-2的下调和Bax的上调，从而增加了Bcl-2 / Bax的比例。总体而言，这些结果表明Sirt1 / Mn-SOD和GRP78 / Caspase-12途径可能与CsV介导的神经保护作用有关。

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