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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Chikusetsu saponin V attenuates H2O2-induced oxidative stress in human neuroblastoma SH-SY5Y cells through Sirt1/PGC-1 alpha/Mn-SOD signaling pathways
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Chikusetsu saponin V attenuates H2O2-induced oxidative stress in human neuroblastoma SH-SY5Y cells through Sirt1/PGC-1 alpha/Mn-SOD signaling pathways

机译：Chikusetsu皂苷V通过Sirt1 / PGC-1 alpha / Mn-SOD信号通路减弱H2O2诱导的人神经母细胞瘤SH-SY5Y细胞的氧化应激。

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摘要

Oxidative stress plays a vital role in the pathogenesis of neurodegenerative diseases. Chikusetsu saponin V (CsV), the most abundant member of saponins from Panax japonicus (SPJ), has attracted increasing attention for its potential to treat neurodegenerative diseases. However, the mechanisms are unclear. Our study intended to investigate the antioxidative effects of CsV in human neuroblastoma SH-SY5Y cells. Our data showed that CsV attenuated H2O2-induced cytotoxicity, inhibited ROS accumulation, increased the activities of superoxide dismutase (SOD) and GSH, and increased mitochondrial membrane potential dose-dependently. Further exploration of the mechanisms showed that CsV exhibited these effects through increasing the activation of oxidative-stress-associated factors including Sirt1, PGC-1 alpha, and Mn-SOD. Moreover, CsV inhibited H2O2-induced down-regulation of Bcl-2 and up-regulation of Bax in a dose-dependent manner and, thus, increased the ratio of Bcl-2/Bax. In conclusion, our study demonstrated that CsV exhibited neuroprotective effects possibly through Sirt1/PGC-1 alpha/Mn-SOD signaling pathways.
机译：氧化应激在神经退行性疾病的发病机理中起着至关重要的作用。 Chikusetsu皂苷V（CsV）是人参（SPJ）中最丰富的皂苷成员，因其治疗神经退行性疾病的潜力而受到越来越多的关注。但是，机制尚不清楚。我们的研究旨在研究CsV在人神经母细胞瘤SH-SY5Y细胞中的抗氧化作用。我们的数据表明，CsV减弱了H2O2诱导的细胞毒性，抑制了ROS的积累，增加了超氧化物歧化酶（SOD）和GSH的活性，并增加了剂量依赖性的线粒体膜电位。对该机制的进一步研究表明，CsV通过增加氧化应激相关因子（包括Sirt1，PGC-1α和Mn-SOD）的活化而显示出这些作用。此外，CsV以剂量依赖的方式抑制了H2O2诱导的Bcl-2的下调和Bax的上调，因此增加了Bcl-2 / Bax的比例。总之，我们的研究表明CsV可能通过Sirt1 / PGC-1 alpha / Mn-SOD信号通路显示出神经保护作用。

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