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首页> 外文期刊>International Journal of Molecular Sciences >Optimal Production and Biochemical Properties of a Lipase from Candida albicans
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Optimal Production and Biochemical Properties of a Lipase from Candida albicans

机译：白念珠菌脂肪酶的最佳生产及生化特性

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摘要

Lipases from microorganisms have multi-faceted properties and play an important role in ever-growing modern biotechnology and, consequently, it is of great significance to develop new ones. In the present work, a lipase gene from Candida albicans (CaLIP10) was cloned and two non-unusual CUG serine codons were mutated into universal codons, and its expression in Pichia pastoris performed optimally, as shown by response surface methodology. Optimal conditions were: initial pH of culture 6.86, temperature 25.53 °C, 3.48% of glucose and 1.32% of yeast extract. The corresponding maximal lipolytic activity of CaLIP10 was 8.06 U/mL. The purified CaLIP10 showed maximal activity at pH 8.0 and 25 °C, and a good resistance to non-ionic surfactants and polar organic solvent was noticed. CaLIP10 could effectively hydrolyze coconut oil, but exhibited no obvious preference to the fatty acids with different carbon length, and diacylglycerol was accumulated in the reaction products, suggesting that CaLIP10 is a potential lipase for the oil industry.
机译：来自微生物的脂肪酶具有多方面的特性，并且在不断发展的现代生物技术中起着重要的作用，因此，开发新的具有重要意义。在目前的工作中，克隆了来自白色念珠菌的脂肪酶基因（CaLIP10），并将两个非寻常的CUG丝氨酸密码子突变为通用密码子，并在毕赤酵母中进行了最佳表达，如响应面法所示。最佳条件为：培养物的初始pH值为6.86，温度为25.53°C，葡萄糖为3.48％，酵母提取物为1.32％。 CaLIP10的相应最大脂解活性为8.06 U / mL。纯化的CaLIP10在pH 8.0和25°C下显示出最大活性，并且对非离子表面活性剂和极性有机溶剂具有良好的耐受性。 CaLIP10可以有效地水解椰子油，但对碳长度不同的脂肪酸没有明显的偏爱，并且在反应产物中积累了二酰基甘油，表明CaLIP10是石油工业的潜在脂肪酶。

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