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首页> 外文期刊>International Journal of Molecular Sciences >Immobilization of Glucose Oxidase in Alginate-Chitosan Microcapsules
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Immobilization of Glucose Oxidase in Alginate-Chitosan Microcapsules

机译：海藻酸盐-壳聚糖微胶囊中葡萄糖氧化酶的固定化

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摘要

In order to improve its stability and catalytic rate in flour, the immobilization of glucose oxidase (GOX) was investigated in this work. The enzyme was encapsulated in calcium alginate-chitosan microspheres (CACM) using an emulsification-internal gelation-GOX adsorption-chitosan coating method. The interaction between alginate and chitosan was confirmed by infrared spectroscopy (IR). The resultant CACM in wet state, whose morphology was investigated by scanning electron microscopy (SEM), was spherical with a mean diameter of about 26 μm. The GOX load, encapsulation efficiency and activity of the CACM-GOX were influenced by concentration of chitosan, encapsulation time and encapsulation pH. The highest total enzymatic activity and encapsulation efficiency was achieved when the pH of the adsorption medium was near the isoelectric point (pI) of GOX, approximately pH 4.0. In addition, the molecular weight of chitosan also evidently influenced the encapsulation efficiency. Storage stabilities of GOX samples were investigated continuously over two months and the retained activity of CACM-GOX was 70.4%, markedly higher than the 7.5% of free enzyme. The results reveal the great potential of CACM-GOX as a flour improver.
机译：为了提高其在面粉中的稳定性和催化速率，在这项工作中研究了葡萄糖氧化酶（GOX）的固定化。使用乳化-内部凝胶化-GOX吸附-壳聚糖包衣法将酶包封在藻酸钙-壳聚糖微球（CACM）中。海藻酸盐和壳聚糖之间的相互作用通过红外光谱法（IR）证实。所得的湿态CACM呈球形，平均直径约为26μm，其处于湿态，其形态通过扫描电子显微镜（SEM）研究。 CACM-GOX的GOX负载，封装效率和活性受壳聚糖浓度，封装时间和封装pH值的影响。当吸附介质的pH值接近GOX的等电点（pI），约为pH 4.0时，可获得最高的总酶促活性和包封效率。另外，壳聚糖的分子量也明显影响包封效率。连续两个月对GOX样品的储存稳定性进行了连续调查，CACM-GOX的保留活性为70.4％，明显高于7.5％的游离酶。结果揭示了CACM-GOX作为面粉改良剂的巨大潜力。

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