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首页> 外文期刊>International Journal of Molecular Sciences >A Cancer-Indicative microRNA Pattern in Normal Prostate Tissue
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A Cancer-Indicative microRNA Pattern in Normal Prostate Tissue

机译：正常前列腺组织中的癌症指示性microRNA模式

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We analyzed the levels of selected micro-RNAs in normal prostate tissue to assess their potential to indicate tumor foci elsewhere in the prostate. Histologically normal prostate tissue samples from 31 prostate cancer patients and two cancer negative control groups with either unsuspicious or elevated prostate specific antigen (PSA) levels (14 and 17 individuals, respectively) were analyzed. Based on the expression analysis of 157 microRNAs in a pool of prostate tissue samples and information from data bases/literature, we selected eight microRNAs for quantification by real-time polymerase chain reactions (RT-PCRs). Selected miRNAs were analyzed in histologically tumor-free biopsy samples from patients and healthy controls. We identified seven microRNAs (miR-124a, miR-146a & b, miR-185, miR-16 and let-7a & b), which displayed significant differential expression in normal prostate tissue from men with prostate cancer compared to both cancer negative control groups. Four microRNAs (miR-185, miR-16 and let-7a and let-7b) remained to significantly discriminate normal tissues from prostate cancer patients from those of the cancer negative control group with elevated PSA levels. The transcript levels of these microRNAs were highly indicative for the presence of cancer in the prostates, independently of the PSA level. Our results suggest a microRNA-pattern in histologically normal prostate tissue, indicating prostate cancer elsewhere in the organ.
机译：我们分析了正常前列腺组织中所选微RNA的水平，以评估其指示前列腺其他部位肿瘤灶的潜力。对来自31名前列腺癌患者和两个癌症阴性对照组的组织学正常的前列腺组织样本进行了分析，这些样本的前列腺特异性抗原（PSA）水平不明确或升高（分别为14和17个人）。基于前列腺组织样本库中157个microRNA的表达分析和来自数据库/文献的信息，我们选择了8个microRNA用于通过实时聚合酶链反应（RT-PCR）进行定量。在来自患者和健康对照的无组织学肿瘤活检样本中分析了选定的miRNA。我们鉴定了七个microRNA（miR-124a，miR-146a和b，miR-185，miR-16和let-7a和b），与两个阴性对照相比，它们在正常前列腺组织中均表现出明显的差异。组。剩下的四个microRNA（miR-185，miR-16和let-7a和let-7b）可以明显地区分前列腺癌患者的正常组织和PSA水平升高的癌症阴性对照组。这些microRNA的转录水平高度指示前列腺癌的存在，与PSA水平无关。我们的结果表明，在组织学正常的前列腺组织中存在microRNA模式，表明该器官其他部位的前列腺癌。

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