...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Molecular Sciences >Development of Collagen/Demineralized Bone Powder Scaffolds and Periosteum-Derived Cells for Bone Tissue Engineering Application
【24h】

Development of Collagen/Demineralized Bone Powder Scaffolds and Periosteum-Derived Cells for Bone Tissue Engineering Application

机译：胶原/脱钙骨粉支架和骨膜衍生细胞的开发在骨组织工程中的应用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to investigate physical and biological properties of collagen (COL) and demineralized bone powder (DBP) scaffolds for bone tissue engineering. DBP was prepared and divided into three groups, based on various particle sizes: 75–125 μm, 125–250 μm, and 250–500 μm. DBP was homogeneously mixed with type I collagen and three-dimensional scaffolds were constructed, applying chemical crosslinking and lyophilization. Upon culture with human periosteum-derived cells (PD cells), osteogenic differentiation of PD cells was investigated using alkaline phosphatase (ALP) activity and calcium assay kits. The physical properties of the COL/DBP scaffolds were obviously different from COL scaffolds, irrespective of the size of DBP. In addition, PD cells cultured with COL scaffolds showed significantly higher cell adhesion and proliferation than those with COL/DBP scaffolds. In contrast, COL/DBP scaffolds exhibited greater osteoinductive potential than COL scaffolds. The PD cells with COL/DBP scaffolds possessed higher ALP activity than those with COL scaffolds. PD cells cultured with COL/DBP scaffolds with 250–500 μm particle size yielded the maximum calcium deposition. In conclusion, PD cells cultured on the scaffolds could exhibit osteoinductive potential. The composite scaffold of COL/DBP with 250–500 μm particle size could be considered a potential bone tissue engineering implant.
机译：这项研究的目的是研究用于骨组织工程的胶原蛋白（COL）和去矿质骨粉（DBP）支架的物理和生物学特性。根据不同的粒径，制备了DBP并将其分为三组：75–125μm，125–250μm和250–500μm。将DBP与I型胶原均匀混合，并通过化学交联和冻干构建三维支架。与人骨膜来源的细胞（PD细胞）培养后，使用碱性磷酸酶（ALP）活性和钙化验试剂盒研究了PD细胞的成骨分化。不论DBP的大小如何，COL / DBP支架的物理性质都与COL支架明显不同。另外，与COL / DBP支架相比，用COL支架培养的PD细胞显示出显着更高的细胞粘附和增殖。相反，COL / DBP支架比COL支架表现出更大的骨诱导潜力。具有COL / DBP支架的PD细胞具有比具有COL / DBP支架的PD细胞更高的ALP活性。用250–500μm粒径的COL / DBP支架培养的PD细胞产生最大的钙沉积。总之，在支架上培养的PD细胞可能显示出骨诱导潜力。 250-500μm粒径的COL / DBP复合支架可以被认为是潜在的骨组织工程植入物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号