...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Molecular Sciences >NOD2 Supports Crypt Survival and Epithelial Regeneration after Radiation-Induced Injury
【24h】

NOD2 Supports Crypt Survival and Epithelial Regeneration after Radiation-Induced Injury

机译：NOD2支持辐射致伤害后的隐窝生存和上皮再生。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) affords stem cell protection and links microbes to intestinal epithelial regeneration. We investigated whether NOD2 status is associated with crypt survival and intestinal epithelial regeneration independent of microbiota-derived molecules. To assess crypt survival, a clonogenic microcolony assay was performed with 15 Gy of X-ray irradiation. The fractional crypt survival rate (46.0 ± 15.5% vs. 24.7 ± 9.2%, p 0.01) and fractional EdU-positive crypt survival rate (29.8 ± 14.5% vs. 9.79 ± 4.37%, p = 0.015) were significantly decreased in the NOD2 ?/? mice compared with the wild-type (WT) mice at 3.5 days after irradiation. To evaluate intestinal epithelial regeneration capability, organoid reconstitution assays were performed. Small bowel crypts of the WT and NOD2 ?/? mice were isolated and seeded into Matrigel for 3D culture. In the organoid reconstitution assays, the number of organoids formed did not differ between the NOD2 ?/? and WT mice. Organoid formation ability was also assessed after exposure to 5 Gy irradiation. Organoid formation ability was significantly decreased in the NOD2 ?/? mice compared with the WT ones after exposure to 5 Gy irradiation (33.2 ± 5.9 vs. 19.7 ± 8.8/well, p 0.01). NOD2 supports crypt survival after potentially lethal irradiation damage and is associated with intestinal epithelial regeneration.
机译：含有核苷酸结合的寡聚域的蛋白质2（NOD2）提供干细胞保护并将微生物与肠道上皮再生联系起来。我们调查了NOD2状态是否与隐窝生存和独立于微生物群的分子的肠上皮再生相关。为了评估隐窝的存活，用15 Gy的X射线进行了克隆形成的微菌落测定。隐窝存活率（46.0±15.5％vs. 24.7±9.2％，p <0.01）和EdU阳性隐窝存活率（29.8±14.5％vs 9.79±4.37％，p = 0.015）显着降低。 NOD2？/？照射后3.5天，将小鼠与野生型（WT）小鼠进行比较。为了评估肠上皮再生能力，进行了类器官重建试验。 WT和NOD2？/？的小肠隐窝。分离小鼠并将其接种到Matrigel中进行3D培养。在类器官重建试验中，形成的类器官数目在NOD2α/β之间没有差异。和野生型小鼠。暴露于5 Gy辐射后，还评估了类器官形成能力。 NOD2α/β中类器官形成能力显着降低。暴露于5 Gy辐射下的小鼠与野生型小鼠相比（33.2±5.9对19.7±8.8 /孔，p <0.01）。 NOD2支持潜在致死性辐射损伤后的隐窝存活，并与肠道上皮再生有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号