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首页> 外文期刊>International Journal of Molecular Sciences >Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant ( Camellia sinensis ) Is Related to the Regulation of Ca 2+ , CaM and Depolarization of Plasma Membrane Potential
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Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant ( Camellia sinensis ) Is Related to the Regulation of Ca 2+ , CaM and Depolarization of Plasma Membrane Potential

机译：茶树中阴离子通道抑制剂NPPB抑制氟的积累与Ca 2+，CaM的调节和血浆膜电位去极化有关

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Tea plant is known to be a hyper-accumulator of fluoride (F). Over-intake of F has been shown to have adverse effects on human health, e.g., dental fluorosis. Thus, understanding the mechanisms fluoride accumulation and developing potential approaches to decrease F uptake in tea plants might be beneficial for human health. In the present study, we found that pretreatment with the anion channel inhibitor NPPB reduced F accumulation in tea plants. Simultaneously, we observed that NPPB triggered Ca 2+ efflux from mature zone of tea root and significantly increased relative CaM in tea roots. Besides, pretreatment with the Ca 2+ chelator (EGTA) and CaM antagonists (CPZ and TFP) suppressed NPPB-elevated cytosolic Ca 2+ fluorescence intensity and CaM concentration in tea roots, respectively. Interestingly, NPPB-inhibited F accumulation was found to be significantly alleviated in tea plants pretreated with either Ca 2+ chelator (EGTA) or CaM antagonists (CPZ and TFP). In addition, NPPB significantly depolarized membrane potential transiently and we argue that the net Ca 2+ and H + efflux across the plasma membrane contributed to the restoration of membrane potential. Overall, our results suggest that regulation of Ca 2+ -CaM and plasma membrane potential depolarization are involved in NPPB-inhibited F accumulation in tea plants.
机译：已知茶树是氟化物（F）的超积累物。已显示过量摄入F对人类健康有不利影响，例如氟中毒。因此，了解氟化物积累的机制并开发减少茶树中F吸收的潜在方法可能对人体健康有益。在本研究中，我们发现用阴离子通道抑制剂NPPB进行预处理可减少茶树中的F积累。同时，我们观察到NPPB触发了Ca 2+从茶根成熟区流出，并显着增加了茶根中的相对CaM。此外，用Ca 2+螯合剂（EGTA）和CaM拮抗剂（CPZ和TFP）预处理分别抑制了NPPB升高的茶树根中胞质Ca 2+荧光强度和CaM浓度。有趣的是，在用Ca 2+螯合剂（EGTA）或CaM拮抗剂（CPZ和TFP）预处理的茶树中，NPPB抑制的F积累被显着减轻。另外，NPPB使膜电位瞬时去极化，我们认为整个质膜上的净Ca​​ 2+和H +外排有助于膜电位的恢复。总体而言，我们的结果表明，Ca 2+ -CaM的调节和质膜电位去极化与茶树中NPPB抑制的F积累有关。

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