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首页> 外文期刊>International Journal of Molecular Sciences >Evaluation of Anthelmintic Activity and Composition of Pumpkin ( Cucurbita pepo L.) Seed Extracts—In Vitro and in Vivo Studies
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Evaluation of Anthelmintic Activity and Composition of Pumpkin ( Cucurbita pepo L.) Seed Extracts—In Vitro and in Vivo Studies

机译：南瓜（南瓜）种子提取物的驱虫活性和成分的评价—体内和体外研究

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A significant number of studies report growing resistance in nematodes thriving in both humans and livestock. This study was conducted to evaluate the in vitro and in vivo anthelmintic efficiency of Curcubita pepo ( C. pepo ) L. hot water extract (HWE), cold water extract (CWE) or ethanol extract (ETE) on two model nematodes: Caenorhabditis elegans ( C. elegans ) and Heligmosoides bakeri ( H. bakeri ). Methods: Raman, IR and LC-MS spectroscopy analyses were performed on the studied plant material to deliver qualitative and quantitative data on the composition of the obtained extracts: ETE, HWE and CWE. The in vitro activity evaluation showed an impact of C. pepo extracts on C. elegans and different developmental stages of H. bakeri . The following in vivo experiments on mice infected with H. bakeri confirmed inhibitory properties of the most active pumpkin extract selected by the in vitro study. All of the extracts were found to contain cucurbitine, aminoacids, fatty acids, and-for the first time-berberine and palmatine were identified. All C. pepo seed extracts exhibited a nematidicidal potential in vitro, affecting the survival of L1 and L2 H. bakeri larvae. The ETE was the strongest and demonstrated a positive effect on H. bakeri eggs hatching and marked inhibitory properties against worm motility, compared to a PBS control. No significant effects of pumpkin seed extracts on C. elegans integrity or motility were found. The EtOH extract in the in vivo studies showed anthelmintic properties against both H. bakeri fecal egg counts and adult worm burdens. The highest egg counts reduction was observed for the 8 g/kg dose (IC 50 against H. bakeri = 2.43; 95% Cl = 2.01–2.94). A decrease in faecal egg counts (FEC) was accompanied by a significant reduction in worm burden of the treated mice compared to the control group. Conclusions: Pumpkin seed extracts may be used to control of Gastrointestinal (G.I.) nematode infections. This relatively inexpensive alternative to the currently available chemotherapeutic should be considered as a novel drug candidate in the nearest future.
机译：大量研究报告说，线虫在人类和牲畜中繁盛的抵抗力不断增强。进行这项研究以评估姜黄对虾（C. pepo）L.热水提取物（HWE），冷水提取物（CWE）或乙醇提取物（ETE）对两种模型线虫的体外和体内驱虫效率：秀丽隐杆线虫（Caenorhabditis elegans） （C. elegans）和Heligmosoides bakeri（H. bakeri）。方法：对所研究的植物材料进行拉曼光谱，IR和LC-MS光谱分析，以提供有关所获得的提取物：ETE，HWE和CWE的成分的定性和定量数据。体外活性评价表明，辣椒提取物对秀丽隐线虫和面包酵母不同发育阶段的影响。接下来的针对面包酵母的小鼠的体内实验证实了通过体外研究选择的最具活性的南瓜提取物的抑制特性。发现所有提取物均含有南瓜碱，氨基酸，脂肪酸，并且首次鉴定出小ber碱和棕榈碱。所有的C. pepo种子提取物在体外均具有杀线虫潜力，影响了L1和L2面包酵母嗜血杆菌幼虫的存活。与PBS对照相比，ETE最强，并显示出对面包酵母H. Bakeri卵孵化具有积极作用，并具有明显的抗蠕虫蠕动特性。没有发现南瓜籽提取物对秀丽隐杆线虫完整性或运动性有显着影响。体内研究中的EtOH提取物显示出针对面包酵母嗜血菌卵计数和成虫蠕虫负担的驱虫特性。对于8 g / kg剂量，观察到的最大卵数减少（针对面包酵母的IC 50 = 2.43; 95％Cl = 2.01-2.94）。与对照组相比，处理过的小鼠的粪便卵数（FEC）减少，蠕虫负担显着减少。结论：南瓜籽提取物可用于控制胃肠道（G.I.）线虫感染。在当前的将来，这种相对便宜的替代物可被认为是新型的候选药物。

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