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首页> 外文期刊>International Journal of Molecular Sciences >Evaluation of Zosteric Acid for Mitigating Biofilm Formation of Pseudomonas putida Isolated from a Membrane Bioreactor System
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Evaluation of Zosteric Acid for Mitigating Biofilm Formation of Pseudomonas putida Isolated from a Membrane Bioreactor System

机译:膜酸对缓解膜生物反应器系统分离恶臭假单胞菌生物膜形成的评价

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This study provides data to define an efficient biocide-free strategy based on zosteric acid to counteract biofilm formation on the membranes of submerged bioreactor system plants. 16S rRNA gene phylogenetic analysis showed that gammaproteobacteria was the prevalent taxa on fouled membranes of an Italian wastewater plant. Pseudomonas was the prevalent genus among the cultivable membrane-fouler bacteria and Pseudomonas putida was selected as the target microorganism to test the efficacy of the antifoulant. Zosteric acid was not a source of carbon and energy for P. putida cells and, at 200 mg/L, it caused a reduction of bacterial coverage by 80%. Biofilm experiments confirmed the compound caused a significant decrease in biomass (−97%) and thickness (−50%), and it induced a migration activity of the peritrichous flagellated P. putida over the polycarbonate surface not amenable to a biofilm phenotype. The low octanol-water partitioning coefficient and the high water solubility suggested a low bioaccumulation potential and the water compartment as its main environmental recipient and capacitor. Preliminary ecotoxicological tests did not highlight direct toxicity effects toward Daphnia magna. For green algae Pseudokirchneriella subcapitata an effect was observed at concentrations above 100 mg/L with a significant growth of protozoa that may be connected to a concurrent algal growth inhibition.
机译:这项研究提供了数据,以定义一种有效的无杀菌剂策略,该策略基于带氢甾酸来抵消淹没式生物反应器系统植物膜上的生物膜形成。 16S rRNA基因系统发育分析表明,γ-变形细菌是意大利污水处理厂污损膜上常见的类群。假单胞菌是可培养的膜污染细菌中的普遍属,恶臭假单胞菌被选为目标微生物以测试该防污剂的功效。带菌酸不是恶臭假单胞菌细胞的碳和能量来源,在200 mg / L时,它导致细菌覆盖率降低了80%。生物膜实验证实,该化合物导致生物量(-97%)和厚度(-50%)显着下降,并且诱导了不适合生物膜表型的周游性鞭毛恶臭假单胞菌在聚碳酸酯表面的迁移活性。低辛醇-水分配系数和高水溶性表明其生物蓄积潜力低,水室是其主要的环境接受者和电容器。初步的生态毒理学测试并未强调对大蚤的直接毒性作用。对于绿藻类次生假单胞菌,在高于100 mg / L的浓度下观察到效果,原生动物显着生长,这可能与同时抑制藻类生长有关。

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