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首页> 外文期刊>International Journal of Molecular Sciences >The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression
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The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression

机译：外显子交界复合体控制参与有丝分裂细胞周期进程的转录子集的有效和忠实的拼接。

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The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.
机译：外显子连接复合物（EJC）沉积在外显子-外显子连接上游的剪接mRNA上，在多种拼接后基因表达事件中发挥重要作用，例如mRNA输出，监视，定位和翻译。但是，尚未完全了解人EJC在mRNA前剪接中的直接作用。使用HeLa细胞，我们耗尽了EJC核心成分之一Y14，并通过深度测序（RNA-Seq）分析了所得的转录组，并通过RT-PCR进行了确认。我们发现Y14是有效和忠实剪接的一组转录本的必需条件，这些转录本富含参与有丝分裂细胞周期进程的短内含子基因。 EJC核心组件（Y14，eIF4AIII或MAGOH）与带有短内含子的模型报告子pre-mRNA的连接显示，这些核心组件是拼接激活的前提条件。两者合计，我们得出结论，组装在前mRNA上的EJC核心对于有丝分裂细胞周期相关基因中短内含子的特定子集的有效和忠实剪接至关重要。

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