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首页> 外文期刊>Emerging Infectious Diseases >Discriminatory Ability of Hypervariable Variable Number Tandem Repeat Loci in Population-based Analysis of Mycobacterium tuberculosis Strains, London, UK
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Discriminatory Ability of Hypervariable Variable Number Tandem Repeat Loci in Population-based Analysis of Mycobacterium tuberculosis Strains, London, UK

机译：基于可变性数串联重复重复基因座的区分能力在基于结核分枝杆菌菌株的人群分析中，英国伦敦

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摘要

To address conflicting results about the stability of vari-able number tandem repeat (VNTR) loci and their value in prospective molecular epidemiology of Mycobacterium tuber-culosis, we conducted a large prospective population-based analysis of all M. tuberculosisstrains in a metropolitan set-ting. Optimal and reproducible conditions for reliable PCR and fragment analysis, comprising enzymes, denaturing con-ditions, and capillary temperature, were identified for a panel of hypervariable loci, including 3232, 2163a, 1982, and 4052. A total of 2,261 individual M. tuberculosisisolates and 265 sets of serial isolates were analyzed by using a standardized 15-loci VNTR panel, then an optimized hypervariable loci panel. The discriminative ability of loci varied substantially; locus VNTR 3232 varied the most, with 19 allelic variants and Hunter-Gaston index value of 0.909 . Hypervariable loci should be included in standardized panels because they can provide consistent comparable results at multiple settings, provided the proposed conditions are adhered to
机译：为了解决关于可变数目串联重复序列（VNTR）基因座的稳定性及其在结核分枝杆菌的前瞻性分子流行病学中的价值的矛盾结果，我们对大城市地区所有结核分枝杆菌菌株进行了基于前瞻性人群的大型分析。婷确定了一组高变位基因的可靠和可靠的PCR和片段分析的最佳条件和可重复条件，包括酶，变性条件和毛细管温度，包括3232、2163a，1982和4052。总共有2261个单独的结核分枝杆菌分离株通过使用标准化的15位VNTR面板，然后是优化的高变位点面板，分析了265套系列分离株。基因座的区分能力差异很大。 VNTR 3232基因座的变异最大，有19个等位基因变异，Hunter-Gaston指数值为0.909。高变位点应包括在标准化面板中，因为只要遵守建议的条件，它们就可以在多个设置下提供一致的可比结果

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