...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >Genotypic effects, maternal effects and grand-maternal effects of immobilized derivatives of the transposable element mariner.
【24h】

Genotypic effects, maternal effects and grand-maternal effects of immobilized derivatives of the transposable element mariner.

机译：转座因子水手的固定衍生物的基因型效应，母体效应和祖母效应。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The baseline rate of spontaneous integration of the autonomous mariner element Mos1 into the germline of Drosophila melanogaster is estimated as 16 +/- 5% (mean +/- SE) among fertile G0 flies. However, the transformation rate is reduced approximately 20-fold in Mos1 constructs with exogenous DNA in the size range 5-12 kb inserted into the SacI site. To provide alternative Mos1 helper plasmids for transformation experiments, two types of Mos1-promoter fusions were constructed: hsp-70:Mos1 and hsp26-Sgs3:Mos1. The former has the Mos1 coding region driven by the hsp70 heat-shock promoter; the latter has it driven by the basal Sgs3 promoter under the control of the hsp26 female-germline specific transcriptional regulator. When introduced into D. melanogaster by P-element-mediated germline transformation, these elements are unable to transpose or excise in the presence of autonomous Mos1-related elements (they are "marooned") because the 5' inverted repeat of Mos1 is missing. As expected, the hsp26-Sgs3:Mos1 fusions exhibit a significantly greater rate of germline excision of a target mariner element than do the hsp70:Mos1 fusions. Unexpectedly, the rate of excision of target mariner elements induced by hsp26-Sgs3:Mos1 is the same in the male germline as in the female germline. Both hsp:Mos1 fusions show strong germline expression and a maternal effect of the mariner transposase. A significant grand-maternal effect of the hsp:Mos1 fusions was also detected as a result of a maternal effect on the germline of the F1 progeny. Among flies carrying the promoter fusions inherited maternally, about three-quarters of the overall rate of germline excision derives from the direct genotypic effect and about one-quarter results from the grand-maternal effect. Despite the strong somatic expression of the hsp:Mos1 fusions, mariner transformants carrying a white+ reporter gene at the SacI site remained stable in the soma.
机译：在可育的G0种蝇中，自主水手元素Mos1自发整合到黑果蝇种系的基线速率估计为16 +/- 5％（平均+/- SE）。但是，在插入到SacI位点的5-12 kb大小的外源DNA中，Mos1构建体的转化率降低了约20倍。为了提供用于转化实验的替代Mos1辅助质粒，构建了两种类型的Mos1启动子融合体：hsp-70：Mos1和hsp26-Sgs3：Mos1。前者具有由hsp70热休克启动子驱动的Mos1编码区。后者在hsp26雌胚种系特异性转录调节子的控制下由基底Sgs3启动子驱动。当通过P元素介导的种系转化将其引入D. melanogaster时，由于缺少Mos1的5'反向重复序列，这些元素在自主Mos1相关元素（被“褐变”）的存在下无法转座或切除。正如预期的那样，与hsp70：Mos1融合相比，hsp26-Sgs3：Mos1融合显示出靶水手的生殖细胞切除率要高得多。出乎意料的是，hsp26-Sgs3：Mos1诱导的目标水手元素的切除率在雄性种系中与雌性种系中相同。两种hsp：Mos1融合体均显示强种系表达和水手转座酶的母体作用。由于对F1后代的种系产生了母体效应，因此还检测到了hsp：Mos1融合体的显着祖母效应。在携带由母体遗传的启动子融合体的果蝇中，种系切除总速度的约四分之三来自直接的基因型效应，而约四分之一的结果来自祖母效应。尽管hsp：Mos1融合蛋白具有强烈的体细胞表达，但在SacI位点携带白色+报告基因的水手转化株在体细胞中仍保持稳定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号