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首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >Identification of a Maize Locus That Modulates the Hypersensitive Defense Response, Using Mutant-Assisted Gene Identification and Characterization
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Identification of a Maize Locus That Modulates the Hypersensitive Defense Response, Using Mutant-Assisted Gene Identification and Characterization

机译：利用突变辅助基因鉴定和表征鉴定调节超敏防御反应的玉米基因座

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Potentially useful naturally occurring genetic variation is often difficult to identify as the effects of individual genes are subtle and difficult to observe. In this study, a novel genetic technique called Mutant-Assisted Gene Identification and Characterization is used to identify naturally occurring loci modulating the hypersensitive defense response (HR) in maize. Mutant-Assisted Gene Identification and Characterization facilitates the identification of naturally occurring alleles underlying phenotypic variation from diverse germplasm, using a mutant phenotype as a “reporter.” In this study the reporter phenotype was caused by a partially dominant autoactive disease resistance gene, Rp1-D21 , which caused HR lesions to form spontaneously all over the plant. Here it is demonstrated that the Rp1-D21 phenotype is profoundly affected by genetic background. By crossing the Rp1-D21 gene into the IBM mapping population, it was possible to map and identify Hrml 1 on chromosome 10, a locus responsible for modulating the HR phenotype conferred by Rp1-D21 . Other loci with smaller effects were identified on chromosomes 1 and 9. These results demonstrate that Mutant-Assisted Gene Identification and Characterization is a viable approach for identifying naturally occurring useful genetic variation.
机译：潜在有用的自然发生的遗传变异通常难以确定，因为单个基因的作用微妙且难以观察。在这项研究中，一种称为突变辅助基因鉴定和表征的新型遗传技术用于鉴定调节玉米中超敏防御反应（HR）的天然基因座。突变辅助基因的鉴定和表征，利用突变表型作为“报告基因”，有助于鉴定来自不同种质表型变异的天然等位基因。在这项研究中，报告基因表型是由部分显性的自发抗病基因Rp1-D21引起的，该基因导致HR损伤在植物中自发形成。在这里证明了Rp1-D21表型受到遗传背景的深刻影响。通过将Rp1-D21基因杂交到IBM定位人群中，可以在10号染色体上定位和鉴定Hrml 1，这是一个负责调节Rp1-D21赋予的HR表型的基因座。在染色体1和9上鉴定出了其他影响较小的基因座。这些结果表明，突变辅助基因的鉴定和表征是鉴定天然存在的有用遗传变异的可行方法。

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