...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >High-resolution mapping of DNase I-hypersensitive sites of Drosophila heat shock genes in Drosophila melanogaster and Saccharomyces cerevisiae.
【24h】

High-resolution mapping of DNase I-hypersensitive sites of Drosophila heat shock genes in Drosophila melanogaster and Saccharomyces cerevisiae.

机译：果蝇热休克基因的果蝇热休克基因的DNase I超敏感位点的高分辨率映射。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

High-resolution analysis of the chromatin structure of the promoter regions of five Drosophila heat shock genes showed a similar location for the hypersensitive sequences relative to the start of transcription. For each of the five genes examined--those coding for hsp27, hsp26, hsp23, hsp70, and hsp83--the DNase I-hypersensitive sites in Drosophila melanogaster nuclei mapped to two regions upstream of the coding region. These sites occurred on the average, 115 and 17 base pairs upstream from the start of transcription of the five heat shock genes examined. This latter site corresponded to sequences at or near the TATA consensus sequence. Sites even further upstream of the hsp27, hsp26, and hsp83 genes were also evident. Additionally, for the two genes examined--hsp70 and hsp83--the DNase I-hypersensitive sites were preserved, at least within this level of resolution (+/- 10 base pairs), when the Drosophila genes were integrated into the Saccharomyces cerevisiae genome. This result indicates that the signals responsible for generating these hypersensitive sites are inherent in the DNA sequences and, in this case, are not highly species specific.
机译：五个果蝇热休克基因的启动子区域的染色质结构的高分辨率分析显示，相对于转录开始，超敏序列的位置相似。对于所检查的五个基因中的每个-编码hsp27，hsp26，hsp23，hsp70和hsp83的基因-果蝇果核中的DNase I超敏位点均位于编码区上游的两个区域。这些位点平均出现在所检查的五个热休克基因转录开始上游的115和17个碱基对的上游。后一个位点对应于TATA共有序列处或附近的序列。在hsp27，hsp26和hsp83基因更上游的位点也很明显。此外，当将果蝇基因整合到酿酒酵母基因组中时，对于所检查的两个基因-hsp70和hsp83-DNase I超敏位点至少在此分辨率水平（+/- 10个碱基对）内得以保留。 。该结果表明，负责产生这些超敏位点的信号在DNA序列中是固有的，在这种情况下，不是高度物种特异性的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号