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首页> 外文期刊>Molecular and Cellular Biology >Relationship between proteins encoded by three human gamma-crystallin genes and distinct polypeptides in the eye lens.
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Relationship between proteins encoded by three human gamma-crystallin genes and distinct polypeptides in the eye lens.

机译：三种人γ-晶状体蛋白基因编码的蛋白质与晶状体中不同多肽之间的关系。

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摘要

Although individual gamma-crystallins from the human eye lens have not been successfully purified and sequenced, most of the genes coding for these lens-specific structural proteins have been cloned and characterized. To investigate the relationship between these genes and the gamma-crystallins of the human lens, we made use of mouse cell lines which contain stably integrated copies of the coding sequences for three of the human gamma-crystallin genes coupled to the human metallothionein IIA promoter. The proteins produced by these hybrid genes in cell culture were detected immunologically and compared by physical characteristics with the gamma-crystallins from the human lens. The protein encoded by the G3 gene showed properties identical to those of the 21,000-molecular-weight gamma-crystallin from 11-month-old lens. The protein isolated from the cells expressing the G4 gene was similar to a 19,000-molecular-weight lens gamma-crystallin, while gene G5 encodes a highly basic gamma-crystallin which may be synthesized in only limited amounts in the human lens. These correlations provide a basis for future investigations on the relationship between putative mutations in human gamma-crystallin genes and altered proteins in hereditary lens cataracts.
机译：尽管来自人眼晶状体的单个γ-晶状体蛋白尚未成功纯化和测序，但已克隆并鉴定了编码这些晶状体特异性结构蛋白的大多数基因。为了研究这些基因与人晶状体的γ-晶状体蛋白之间的关系，我们利用了小鼠细胞系，该细胞系包含与人金属硫蛋白IIA启动子偶联的三个人γ-晶状体蛋白基因的编码序列的稳定整合拷贝。这些杂交基因在细胞培养物中产生的蛋白质通过免疫学检测，并通过物理特征与人晶状体中的γ-晶状体蛋白进行比较。 G3基因编码的蛋白质显示出与11个月大的晶体中21,000分子量的γ-晶状体蛋白相同的特性。从表达G4基因的细胞中分离出的蛋白质类似于19,000分子量的晶状体γ-晶状体蛋白，而基因G5编码一种高度碱性的γ-晶状体蛋白，在人晶状体中只能以有限的量合成。这些相关性为将来研究人类γ-晶状体蛋白基因的假定突变与遗传性晶状体白内障中蛋白质改变之间的关系提供了基础。

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