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首页> 外文期刊>Molecular and Cellular Biology >Identification of sequence elements that confer cell-type-specific control of MF alpha 1 expression in Saccharomyces cerevisiae.
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Identification of sequence elements that confer cell-type-specific control of MF alpha 1 expression in Saccharomyces cerevisiae.

机译：鉴定赋予酿酒酵母MFα1表达的细胞类型特异性控制的序列元件。

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摘要

The MF alpha 1 gene of Saccharomyces cerevisiae, a major structural gene for mating pheromone alpha factor, is an alpha-specific gene whose expression is regulated by the mating-type locus. To study the role of sequences upstream of MF alpha 1 in its expression and regulation, we generated two sets of promoter deletions: upstream deletions and internal deletions. By analyzing these deletions, we have identified a TATA box and two closely related, tandemly arranged upstream activation sites as necessary elements for MF alpha 1 expression. Two upstream activation sites were located ca. 300 and 250 base pairs upstream of the MF alpha 1 transcription start points, which were also determined in this study. Each site contained a homologous 22-base-pair sequence, and both sites were required for maximum transcription level. The distance between the upstream activation sites and the transcription start points could be altered without causing loss of transcription efficiency, and the sites were active in either orientation with respect to the coding region. These elements conferred cell type-specific expression on a heterologous promoter. Analysis with host mating-type locus mutants indicates that these sequences are the sites through which the MAT alpha 1 product exerts its action to activate the MF alpha 1 gene. Homologous sequences with these elements were found in other alpha-specific genes, MF alpha 2 and STE3, and may mediate activation of this set of genes by MAT alpha 1.
机译：酿酒酵母的MF alpha 1基因是交配信息素α因子的主要结构基因，是一种α特异性基因，其表达受交配型基因座调控。为了研究MF alpha 1上游序列在其表达和调控中的作用，我们产生了两组启动子缺失：上游缺失和内部缺失。通过分析这些删除，我们确定了一个TATA框和两个紧密相关，串联排列的上游激活位点，作为MF alpha 1表达的必要元素。大约两个上游激活位点位于。 MF alpha 1转录起始点上游的300和250个碱基对，也已在本研究中确定。每个位点都包含一个同源的22个碱基对的序列，并且两个位点都是最大转录水平所必需的。上游活化位点和转录起始点之间的距离可以改变而不会引起转录效率的损失，并且这些位点在相对于编码区的任一方向上都是有活性的。这些元件赋予异源启动子细胞类型特异性表达。宿主交配型基因座突变体的分析表明，这些序列是MATα1产物通过其发挥作用激活MFα1基因的位点。在其他alpha特异性基因MF alpha 2和STE3中发现了具有这些元素的同源序列，并且可能通过MAT alpha 1介导了这组基因的激活。

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