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首页> 外文期刊>Molecular and Cellular Biology >Regulation of histone and beta A-globin gene expression during differentiation of chicken erythroid cells.
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Regulation of histone and beta A-globin gene expression during differentiation of chicken erythroid cells.

机译：鸡红系细胞分化过程中组蛋白和βA-球蛋白基因表达的调节。

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The expression of the genes for several histones and beta A-globin was examined in the chicken erythroid cells lineage. During the transition from CFU-(E) to the mature erythrocyte, histone H5 gradually increased fourfold in nuclei with little concomitant displacement of the H1 histones. This resulted in a 70% net increase in linker histone (H1 plus H5) content. The differential accumulation of H5 reflected (i) an increase in the transcriptional activity of the H5 gene occurring at the erythroblast stage, (ii) an apparent longer half-life of H5 mRNA, and (iii) a higher stability of the protein. Although the transcriptional activity of the histone genes (except H5) decreased with cell age, it was not tightly coupled to the S phase. On the other hand, the mRNA levels for these histones were tightly regulated during the cell cycle. Use of protein and DNA synthesis inhibitors indicated that the content of H5 mRNA was regulated at the posttranscriptional level by a control mechanism(s) differing from those for the other histones. Although the transcription rates of the H5 and beta A-globin genes were comparable, differential accumulation of beta A-globin mRNA led to a 30- to 170-fold-higher copy number of the beta A-globin mRNA as the cell matured.
机译：在鸡红系细胞谱系中检查了几种组蛋白和βA-球蛋白的基因表达。在从CFU-（E）转变为成熟的红细胞的过程中，组蛋白H5在细胞核中逐渐增加了四倍，而伴随的H1组蛋白移位却很少。这导致接头组蛋白（H1 + H5）含量的净增加了70％。 H5的差异积累反映了（i）在成红细胞阶段发生的H5基因转录活性的增加;（ii）H5 mRNA明显更长的半衰期;以及（iii）蛋白质的更高稳定性。尽管组蛋白基因（H5除外）的转录活性随细胞年龄而降低，但它与S期并没有紧密结合。另一方面，这些组蛋白的mRNA水平在细胞周期中受到严格调节。蛋白质和DNA合成抑制剂的使用表明，H5 mRNA的含量在转录后水平受到与其他组蛋白不同的控制机制的调节。尽管H5和βA球蛋白基因的转录速率相当，但是随着细胞的成熟，βA球蛋白mRNA的差异积累导致βA球蛋白mRNA的拷贝数增加了30到170倍。

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