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首页> 外文期刊>Molecular and Cellular Biology >Control of yeast gene expression by transposable elements: maximum expression requires a functional Ty activator sequence and a defective Ty promoter.
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Control of yeast gene expression by transposable elements: maximum expression requires a functional Ty activator sequence and a defective Ty promoter.

机译：通过转座因子控制酵母基因表达：最大表达需要功能性Ty激活序列和有缺陷的Ty启动子。

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Integration of a transposable element adjacent to a gene frequently results in an alteration in expression of the nearby gene. The purpose of our experiments was to identify cis-acting sequences within a yeast transposon (Ty) that are important for expression of the adjacent gene. The role of these sequences in Ty transcription was also analyzed in order to examine the relationship between Ty and adjacent gene expression. Three naturally occurring Ty elements located at the HIS4 locus were examined. These Ty elements differed by multiple sequence changes and had different effects on HIS4 expression. To determine which sequences were important to Ty and HIS4 expression, Ty::lacZ and Ty::HIS4::lacZ fusion genes were constructed and analyzed. Results of these experiments indicated that a sequence element is present in the Ty epsilon region that is necessary for HIS4 expression but which has only a modest effect on Ty transcription. Additionally, a mutation in the Ty promoter region decreased Ty transcription and increased HIS4 expression. The opposite effects of this mutation on Ty and adjacent gene expression were probably caused by promoter competition.
机译：与基因相邻的转座因子的整合经常导致附近基因表达的改变。我们实验的目的是鉴定酵母转座子（Ty）中对相邻基因表达很重要的顺式作用序列。还分析了这些序列在Ty转录中的作用，以检查Ty和邻近基因表达之间的关系。检查了位于HIS4基因座的三个天然Ty元素。这些Ty元素因多个序列变化而不同，并且对HIS4表达具有不同的影响。为了确定哪些序列对Ty和HIS4表达重要，构建并分析了Ty :: lacZ和Ty :: HIS4 :: lacZ融合基因。这些实验的结果表明，在Ty epsilon区域中存在一个序列元件，该序列元件对于HIS4表达是必需的，但是对Ty转录仅具有中等作用。此外，Ty启动子区域的突变降低了Ty的转录并增加了HIS4表达。该突变对Ty和邻近基因表达的相反作用可能是由启动子竞争引起的。

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