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首页> 外文期刊>Molecular and Cellular Biology >RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination.
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RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination.

机译：RAD1，酿酒酵母的切除修复基因，也参与重组。

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The RAD1 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of damaged DNA. In this paper, we report our observations on the effect of the RAD1 gene on genetic recombination. Mitotic intrachromosomal and interchromosomal recombination in RAD+, rad1, rad52, and other rad mutant strains was examined. The rad1 deletion mutation and some rad1 point mutations reduced the frequency of intrachromosomal recombination of a his3 duplication, in which one his3 allele is deleted at the 3' end while the other his3 allele is deleted at the 5' end. Mutations in the other excision repair genes, RAD2, RAD3, and RAD4, did not lower recombination frequencies in the his3 duplication. As expected, recombination between the his3 deletion alleles in the duplication was reduced in the rad52 mutant. The frequency of HIS3+ recombinants fell synergistically in the rad1 rad52 double mutant, indicating that the RAD1 and RAD52 genes affect this recombination via different pathways. In contrast to the effect of mutations in the RAD52 gene, mutations in the RAD1 gene did not lower intrachromosomal and interchromosomal recombination between heteroalleles that carry point mutations rather than partial deletions; however, the rad1 delta mutation did lower the frequency of integration of linear plasmids and DNA fragments into homologous genomic sequences. We suggest that RAD1 plays a role in recombination after the formation of the recombinogenic substrate.
机译：啤酒酵母的RAD1基因是切除修复受损DNA的切口步骤所必需的。在本文中，我们报告了我们对RAD1基因对基因重组的影响的观察结果。检查了RAD +，rad1，rad52和其他rad突变菌株中的有丝分裂染色体内和染色体间重组。 rad1缺失突变和一些rad1点突变降低了his3复制的染色体内重组频率，其中一个his3等位基因在3'端缺失，而另一个his3等位基因在5'端缺失。其他切除修复基因RAD2，RAD3和RAD4中的突变并未降低his3复制中的重组频率。如所期望的，在rad52突变体中，在重复中的his3缺失等位基因之间的重组减少了。 HIS3 +重组体的频率在rad1 rad52双重突变体中协同下降，表明RAD1和RAD52基因通过不同途径影响这种重组。与RAD52基因突变的影响相反，RAD1基因突变不会降低携带点突变而不是部分缺失的异等位基因之间的染色体内和染色体间重组。但是，rad1 delta突变确实降低了线性质粒和DNA片段整合入同源基因组序列的频率。我们建议RAD1在重组底​​物形成后在重组中发挥作用。

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