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首页> 外文期刊>Molecular and Cellular Biology >Mitochondrial DNA rearrangements and transcriptional alterations in the male-sterile cytoplasm of Ogura radish.
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Mitochondrial DNA rearrangements and transcriptional alterations in the male-sterile cytoplasm of Ogura radish.

机译：小仓萝卜雄性不育细胞质中的线粒体DNA重排和转录改变。

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摘要

Maternally inherited mutations, such as cytoplasmic male sterility, provide useful systems in which to study the function of plant mitochondrial genomes and also their interaction with nuclear genes. We have studied the organization and expression of the organelle genomes of the male-sterile cytoplasm of Ogura radish and compared them with those of normal radish to identify alterations that might be involved in cytoplasmic male sterility. The chloroplast DNAs of Ogura and normal radish are virtually indistinguishable, whereas their mitochondrial DNAs are highly rearranged. Alignment of a restriction map constructed for the 257-kilobase Ogura mitochondrial genome with that published for the 242-kilobase genome of normal radish reveals that the two mitochondrial DNAs differ in arrangement by at least 10 inversions. The transcriptional patterns of several known mitochondrial genes and of rearranged mitochondrial sequences were examined in three nuclear backgrounds. Altered transcripts were observed for three mitochondrial genes, atpA, atp6, and coxI. Rearrangements map near each of these genes and therefore may be responsible for their transcriptional alterations. Radish nuclear genes that restore fertility to the Ogura cytoplasm have no effect on the atp6 and coxI transcripts, but do influence the atpA transcriptional pattern.
机译：母体遗传的突变，例如细胞质雄性不育，提供了有用的系统，可用于研究植物线粒体基因组的功能以及它们与核基因的相互作用。我们已经研究了小仓萝卜雄性不育细胞质的细胞器基因组的组织和表达，并将其与正常萝卜的细胞质基因组进行比较，以确定可能与胞质雄性不育有关的改变。小仓和正常萝卜的叶绿体DNA几乎没有区别，而线粒体DNA高度重排。为257碱基的Ogura线粒体基因组构建的限制性图谱与正常萝卜的242碱基的基因组构建的限制性图谱的比对揭示，两个线粒体DNA的排列方式至少相差10个倒位。在三个核背景下检查了几个已知的线粒体基因和重排的线粒体序列的转录模式。观察到三个线粒体基因atpA，atp6和coxI的转录物发生了变化。重排位于这些基因中的每一个附近，因此可能是其转录改变的原因。恢复小仓质质繁殖力的萝卜核基因对atp6和coxI转录本没有影响，但确实会影响atpA转录模式。

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