...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Regulation of expression and activity of the yeast transcription factor ADR1.
【24h】

Regulation of expression and activity of the yeast transcription factor ADR1.

机译：调节酵母转录因子ADR1的表达和活性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Disruption of ADR1, a positive regulatory gene in the yeast Saccharomyces cerevisiae, abolished derepression of ADH2 but did not affect glucose repression of ADH2 or cell viability. The ADR1 mRNA was 5 kilobases long and had an unusually long leader containing 509 nucleotides. ADR1 mRNA levels were regulated by the carbon source in a strain-dependent fashion. beta-Galactosidase levels measured in strains carrying an ADR1-lacZ gene fusion paralleled ADR1 and ADR1-lacZ mRNA levels, indicating a lack of translational regulation of ADR1 mRNA. ADH2 was regulated by the carbon source to the same extent in all strains examined and showed complete dependence on ADR1 as well. The expression of ADR1 mRNA and an ADR1-beta-galactosidase fusion protein during glucose repression suggested that the activity of the ADR1 protein is regulated at the posttranslational level to properly regulate ADH2 expression. The ADR1-beta-galactosidase fusion protein was able to activate ADH2 expression during glucose repression but showed significantly higher levels of activation upon derepression. A similar result was obtained when ADR1 was present on a multicopy plasmid. These results suggest that low-level expression of ADR1 is required to maintain glucose repression of ADH2 and are consistent with the hypothesis that ADR1 is regulated at the posttranslational level.
机译：酵母酿酒酵母中的阳性调控基因ADR1的破坏消除了ADH2的抑制，但并未影响ADH2的葡萄糖抑制或细胞活力。 ADR1 mRNA的长度为5千个碱基，其前导序列异常长，包含509个核苷酸。碳源以应变依赖的方式调节ADR1 mRNA的水平。在携带ADR1-lacZ基因融合体的菌株中测得的β-半乳糖苷酶水平与ADR1和ADR1-lacZ mRNA水平平行，这表明ADR1 mRNA缺乏翻译调控。在所有检测的菌株中，ADH2受碳源的调节程度相同，并且也完全依赖于ADR1。葡萄糖阻抑过程中ADR1 mRNA和ADR1-β-半乳糖苷酶融合蛋白的表达表明，ADR1蛋白的活性在翻译后水平受到调节，以正确调节ADH2的表达。 ADR1-β-半乳糖苷酶融合蛋白能够在葡萄糖阻抑过程中激活ADH2表达，但在阻抑后显示出明显更高的激活水平。当ADR1存在于多拷贝质粒上时，可获得相似的结果。这些结果表明，维持ADH2的葡萄糖抑制需要ADR1的低水平表达，并且与ADR1在翻译后水平受调控的假设相一致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号