• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Functional distinctions between yeast TATA elements.
【24h】

Functional distinctions between yeast TATA elements.

机译：酵母TATA元素之间的功能区别。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although the yeast his3 promoter region contains two functional TATA elements, TR and TC, the GCN4 and GAL4 upstream activator proteins stimulate transcription only through TR. In combination with GAL4, an oligonucleotide containing the sequence TATAAA is fully sufficient for TR function, whereas almost all single-base-pair substitutions of this sequence abolish the ability of this element to activate transcription. Further analysis of these and other mutations of the TR element led to the following conclusions. First, sequences downstream of the TATAAA sequence are important for TR function. Second, a double mutant, TATTTA, can serve as a TR element even though the corresponding single mutation, TATTAA, is unable to do so. Third, three mutations have the novel property of being able to activate transcription in combination with GCN4 but not with GAL4; this finding suggests that activation by GCN4 and by GAL4 may not occur by identical mechanisms. From these observations, we address the question of whether there is a single TATA-binding factor required for the transcription of all genes.
机译：尽管酵母his3启动子区域包含两个功能性TATA元素TR和TC，但GCN4和GAL4上游激活蛋白仅通过TR刺激转录。与GAL4结合使用时，含有序列TATAAA的寡核苷酸足以实现TR功能，而该序列的几乎所有单碱基对取代都消除了该元件激活转录的能力。对TR元件的这些和其他突变的进一步分析得出以下结论。首先，TATAAA序列下游的序列对于TR功能很重要。其次，即使相应的单个突变体TATTAA无法做到，双重突变体TATTTA也可以充当TR元件。第三，三个突变具有与GCN4结合但与GAL4结合不能激活转录的新颖特性。这一发现表明，由GCN4和GAL4激活可能不会通过相同的机制发生。从这些观察中，我们解决了所有基因转录是否需要单个TATA结合因子的问题。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号